Chromosome localization and expression pattern of Lmyc and Bmyc in murine embryonal carcinoma cells

S. Ingvarsson, S. Sundaresan, P. Jin, U. Francke, C. Asker, Janos Sumegi, G. Klein, T. Sejersen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Using Southern blot analysis of DNA from mouse-hamster somatic cell hybrids, we have mapped Lmyc and Bmyc, two members of the myc family of genes, to mouse chromosomes 4 and 2, respectively. Furthermore, we have compared the regulation of Lmyc and Bmyc expression under different growth conditions and during in vitro differentiation of the murine EC line F9 and considered the findings in relation to our previous studies on Nmyc and c-myc expression in the same line (Sejersen et al., 1987). Lmyc was down-regulated at an early stage of visceral endoderm differentiation, similarly to c-myc and Nmyc, while Bmyc was expressed at a constant low level at all stages. Lmyc, but not c-myc and Nmyc, was upregulated in terminally differentiated visceral endoderm cells. Inhibition of protein synthesis by cycloheximide for 4 h induced a 70% increase in Lmyc and 30% increase in Bmyc transcript levels, indicating that the expression of these genes is negatively regulated by a short-lived protein. Mitogenic stimulation with insulin and transferrin did not affect Lmyc and Bmyc mRNA levels. Lmyc transcripts have a half life of 30 min, whereas the Bmyc transcript is highly stable, with a half life of 6 h. The half-lives of the c-myc and Nmyc transcripts have been estimated previously as 40 and 130 min, respectively.

Original languageEnglish (US)
Pages (from-to)679-685
Number of pages7
JournalOncogene
Volume3
Issue number6
StatePublished - Dec 1 1988

Fingerprint

Embryonal Carcinoma Stem Cells
Endoderm
Half-Life
Chromosomes
Chromosomes, Human, Pair 4
myc Genes
Chromosomes, Human, Pair 2
Hybrid Cells
Cycloheximide
Transferrin
Southern Blotting
Cricetinae
Proteins
Insulin
Gene Expression
Messenger RNA
DNA
Growth
Inhibition (Psychology)
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Ingvarsson, S., Sundaresan, S., Jin, P., Francke, U., Asker, C., Sumegi, J., ... Sejersen, T. (1988). Chromosome localization and expression pattern of Lmyc and Bmyc in murine embryonal carcinoma cells. Oncogene, 3(6), 679-685.

Chromosome localization and expression pattern of Lmyc and Bmyc in murine embryonal carcinoma cells. / Ingvarsson, S.; Sundaresan, S.; Jin, P.; Francke, U.; Asker, C.; Sumegi, Janos; Klein, G.; Sejersen, T.

In: Oncogene, Vol. 3, No. 6, 01.12.1988, p. 679-685.

Research output: Contribution to journalArticle

Ingvarsson, S, Sundaresan, S, Jin, P, Francke, U, Asker, C, Sumegi, J, Klein, G & Sejersen, T 1988, 'Chromosome localization and expression pattern of Lmyc and Bmyc in murine embryonal carcinoma cells', Oncogene, vol. 3, no. 6, pp. 679-685.
Ingvarsson S, Sundaresan S, Jin P, Francke U, Asker C, Sumegi J et al. Chromosome localization and expression pattern of Lmyc and Bmyc in murine embryonal carcinoma cells. Oncogene. 1988 Dec 1;3(6):679-685.
Ingvarsson, S. ; Sundaresan, S. ; Jin, P. ; Francke, U. ; Asker, C. ; Sumegi, Janos ; Klein, G. ; Sejersen, T. / Chromosome localization and expression pattern of Lmyc and Bmyc in murine embryonal carcinoma cells. In: Oncogene. 1988 ; Vol. 3, No. 6. pp. 679-685.
@article{a05b3f6003f34ca69beea70cbb4d6377,
title = "Chromosome localization and expression pattern of Lmyc and Bmyc in murine embryonal carcinoma cells",
abstract = "Using Southern blot analysis of DNA from mouse-hamster somatic cell hybrids, we have mapped Lmyc and Bmyc, two members of the myc family of genes, to mouse chromosomes 4 and 2, respectively. Furthermore, we have compared the regulation of Lmyc and Bmyc expression under different growth conditions and during in vitro differentiation of the murine EC line F9 and considered the findings in relation to our previous studies on Nmyc and c-myc expression in the same line (Sejersen et al., 1987). Lmyc was down-regulated at an early stage of visceral endoderm differentiation, similarly to c-myc and Nmyc, while Bmyc was expressed at a constant low level at all stages. Lmyc, but not c-myc and Nmyc, was upregulated in terminally differentiated visceral endoderm cells. Inhibition of protein synthesis by cycloheximide for 4 h induced a 70{\%} increase in Lmyc and 30{\%} increase in Bmyc transcript levels, indicating that the expression of these genes is negatively regulated by a short-lived protein. Mitogenic stimulation with insulin and transferrin did not affect Lmyc and Bmyc mRNA levels. Lmyc transcripts have a half life of 30 min, whereas the Bmyc transcript is highly stable, with a half life of 6 h. The half-lives of the c-myc and Nmyc transcripts have been estimated previously as 40 and 130 min, respectively.",
author = "S. Ingvarsson and S. Sundaresan and P. Jin and U. Francke and C. Asker and Janos Sumegi and G. Klein and T. Sejersen",
year = "1988",
month = "12",
day = "1",
language = "English (US)",
volume = "3",
pages = "679--685",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Chromosome localization and expression pattern of Lmyc and Bmyc in murine embryonal carcinoma cells

AU - Ingvarsson, S.

AU - Sundaresan, S.

AU - Jin, P.

AU - Francke, U.

AU - Asker, C.

AU - Sumegi, Janos

AU - Klein, G.

AU - Sejersen, T.

PY - 1988/12/1

Y1 - 1988/12/1

N2 - Using Southern blot analysis of DNA from mouse-hamster somatic cell hybrids, we have mapped Lmyc and Bmyc, two members of the myc family of genes, to mouse chromosomes 4 and 2, respectively. Furthermore, we have compared the regulation of Lmyc and Bmyc expression under different growth conditions and during in vitro differentiation of the murine EC line F9 and considered the findings in relation to our previous studies on Nmyc and c-myc expression in the same line (Sejersen et al., 1987). Lmyc was down-regulated at an early stage of visceral endoderm differentiation, similarly to c-myc and Nmyc, while Bmyc was expressed at a constant low level at all stages. Lmyc, but not c-myc and Nmyc, was upregulated in terminally differentiated visceral endoderm cells. Inhibition of protein synthesis by cycloheximide for 4 h induced a 70% increase in Lmyc and 30% increase in Bmyc transcript levels, indicating that the expression of these genes is negatively regulated by a short-lived protein. Mitogenic stimulation with insulin and transferrin did not affect Lmyc and Bmyc mRNA levels. Lmyc transcripts have a half life of 30 min, whereas the Bmyc transcript is highly stable, with a half life of 6 h. The half-lives of the c-myc and Nmyc transcripts have been estimated previously as 40 and 130 min, respectively.

AB - Using Southern blot analysis of DNA from mouse-hamster somatic cell hybrids, we have mapped Lmyc and Bmyc, two members of the myc family of genes, to mouse chromosomes 4 and 2, respectively. Furthermore, we have compared the regulation of Lmyc and Bmyc expression under different growth conditions and during in vitro differentiation of the murine EC line F9 and considered the findings in relation to our previous studies on Nmyc and c-myc expression in the same line (Sejersen et al., 1987). Lmyc was down-regulated at an early stage of visceral endoderm differentiation, similarly to c-myc and Nmyc, while Bmyc was expressed at a constant low level at all stages. Lmyc, but not c-myc and Nmyc, was upregulated in terminally differentiated visceral endoderm cells. Inhibition of protein synthesis by cycloheximide for 4 h induced a 70% increase in Lmyc and 30% increase in Bmyc transcript levels, indicating that the expression of these genes is negatively regulated by a short-lived protein. Mitogenic stimulation with insulin and transferrin did not affect Lmyc and Bmyc mRNA levels. Lmyc transcripts have a half life of 30 min, whereas the Bmyc transcript is highly stable, with a half life of 6 h. The half-lives of the c-myc and Nmyc transcripts have been estimated previously as 40 and 130 min, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0024204928&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024204928&partnerID=8YFLogxK

M3 - Article

VL - 3

SP - 679

EP - 685

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 6

ER -