Chromatographic studies of protein-based chiral separations

Cong Bi, Xiwei Zheng, Shiden Azaria, Sandya Beeram, Zhao Li, David S. Hage

Research output: Contribution to journalReview article

13 Scopus citations

Abstract

The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha1-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations.

Original languageEnglish (US)
Article number27
JournalSeparations
Volume3
Issue number3
DOIs
StatePublished - Sep 2016

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Keywords

  • Chiral high-performance liquid chromatography (HPLC)
  • Chiral recognition
  • Chromatographic studies of drug-protein interactions
  • Frontal analysis
  • Protein-based chiral stationary phases
  • Zonal elution

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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