Chondrocyte primary cilium is mechanosensitive and responds to low-intensity-ultrasound by altering its length and orientation

Anuradha Subramanian, Gaurav Budhiraja, Neety Sahu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The primary cilium has been implicated in mechanotransduction, however, its mechanosensory role in transducing signals under low-intensity ultrasound (LIUS) which has the potential to repair fractures and cartilage, remains uninvestigated. This study examined the impact of continuous-wave US, at the cell resonance frequency of 5.0 MHz and pressure amplitude of either 14 or 60 kPa, on the incidence, length and orientation of primary cilium in bovine articular chondrocytes. Visualization of primary cilium with acetylated α-tubulin staining demonstrated that the primary cilium was elongated, bent under US and these changes were reversible. Basal expression of phospho-ERK1/2 was lower in deciliated chondrocytes, thus implicating the role for the primary cilium in transducing signals via the MAPK/ERK pathway. This study demonstrates that the chondrocyte primary cilium is mechanosensitive and responds to US by altering its length and orientation.

Original languageEnglish (US)
Pages (from-to)60-64
Number of pages5
JournalInternational Journal of Biochemistry and Cell Biology
Volume91
DOIs
StatePublished - Oct 2017

Fingerprint

Cilia
Cartilage
Tubulin
Chondrocytes
Repair
Visualization
Ultrasonics
Cartilage Fractures
MAP Kinase Signaling System
Joints
Staining and Labeling
Pressure
Incidence

Keywords

  • Chondrocytes
  • Extracellular-regulated kinase (ERK)
  • Mechanotransduction
  • Primary cilium
  • Ultrasound(US)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

@article{2d4b846c70ac456b99e25c2840c4f3ae,
title = "Chondrocyte primary cilium is mechanosensitive and responds to low-intensity-ultrasound by altering its length and orientation",
abstract = "The primary cilium has been implicated in mechanotransduction, however, its mechanosensory role in transducing signals under low-intensity ultrasound (LIUS) which has the potential to repair fractures and cartilage, remains uninvestigated. This study examined the impact of continuous-wave US, at the cell resonance frequency of 5.0 MHz and pressure amplitude of either 14 or 60 kPa, on the incidence, length and orientation of primary cilium in bovine articular chondrocytes. Visualization of primary cilium with acetylated α-tubulin staining demonstrated that the primary cilium was elongated, bent under US and these changes were reversible. Basal expression of phospho-ERK1/2 was lower in deciliated chondrocytes, thus implicating the role for the primary cilium in transducing signals via the MAPK/ERK pathway. This study demonstrates that the chondrocyte primary cilium is mechanosensitive and responds to US by altering its length and orientation.",
keywords = "Chondrocytes, Extracellular-regulated kinase (ERK), Mechanotransduction, Primary cilium, Ultrasound(US)",
author = "Anuradha Subramanian and Gaurav Budhiraja and Neety Sahu",
year = "2017",
month = "10",
doi = "10.1016/j.biocel.2017.08.018",
language = "English (US)",
volume = "91",
pages = "60--64",
journal = "International Journal of Biochemistry and Cell Biology",
issn = "1357-2725",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Chondrocyte primary cilium is mechanosensitive and responds to low-intensity-ultrasound by altering its length and orientation

AU - Subramanian, Anuradha

AU - Budhiraja, Gaurav

AU - Sahu, Neety

PY - 2017/10

Y1 - 2017/10

N2 - The primary cilium has been implicated in mechanotransduction, however, its mechanosensory role in transducing signals under low-intensity ultrasound (LIUS) which has the potential to repair fractures and cartilage, remains uninvestigated. This study examined the impact of continuous-wave US, at the cell resonance frequency of 5.0 MHz and pressure amplitude of either 14 or 60 kPa, on the incidence, length and orientation of primary cilium in bovine articular chondrocytes. Visualization of primary cilium with acetylated α-tubulin staining demonstrated that the primary cilium was elongated, bent under US and these changes were reversible. Basal expression of phospho-ERK1/2 was lower in deciliated chondrocytes, thus implicating the role for the primary cilium in transducing signals via the MAPK/ERK pathway. This study demonstrates that the chondrocyte primary cilium is mechanosensitive and responds to US by altering its length and orientation.

AB - The primary cilium has been implicated in mechanotransduction, however, its mechanosensory role in transducing signals under low-intensity ultrasound (LIUS) which has the potential to repair fractures and cartilage, remains uninvestigated. This study examined the impact of continuous-wave US, at the cell resonance frequency of 5.0 MHz and pressure amplitude of either 14 or 60 kPa, on the incidence, length and orientation of primary cilium in bovine articular chondrocytes. Visualization of primary cilium with acetylated α-tubulin staining demonstrated that the primary cilium was elongated, bent under US and these changes were reversible. Basal expression of phospho-ERK1/2 was lower in deciliated chondrocytes, thus implicating the role for the primary cilium in transducing signals via the MAPK/ERK pathway. This study demonstrates that the chondrocyte primary cilium is mechanosensitive and responds to US by altering its length and orientation.

KW - Chondrocytes

KW - Extracellular-regulated kinase (ERK)

KW - Mechanotransduction

KW - Primary cilium

KW - Ultrasound(US)

UR - http://www.scopus.com/inward/record.url?scp=85028927312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028927312&partnerID=8YFLogxK

U2 - 10.1016/j.biocel.2017.08.018

DO - 10.1016/j.biocel.2017.08.018

M3 - Article

C2 - 28870737

AN - SCOPUS:85028927312

VL - 91

SP - 60

EP - 64

JO - International Journal of Biochemistry and Cell Biology

JF - International Journal of Biochemistry and Cell Biology

SN - 1357-2725

ER -