Cholesterol secosterol aldehyde adduction and aggregation of Cu,Zn-superoxide dismutase

Potential implications in ALS

Lucas S. Dantas, Adriano B. Chaves-Filho, Fernando R. Coelho, Thiago Cardoso Genaro De Mattos, Keri A. Tallman, Ned A. Porter, Ohara Augusto, Sayuri Miyamoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by degeneration of upper and lower motor neurons. While the fundamental causes of the disease are still unclear, the accumulation of Cu,Zn-superoxide dismutase (SOD1) immunoreactive aggregates is associated with familial ALS cases. Cholesterol 5,6-secosterol aldehydes (Seco A and Seco B) are reported to contribute to neurodegenerative disease pathology by inducing protein modification and aggregation. Here we have investigated the presence of secosterol aldehydes in ALS SOD1-G93A rats and their capacity to induce SOD1 aggregation. Secosterol aldehydes were analyzed in blood plasma, spinal cord and motor cortex of ALS rats at the pre-symptomatic and symptomatic stages. Seco B was significantly increased in plasma of symptomatic ALS rats compared to pre-symptomatic animals, suggesting an association with disease progression. In vitro experiments showed that both Seco A and Seco B induce the formation of high molecular weight (HMW) SOD1 aggregates with amorphous morphology. SOD1 adduction to ω-alkynyl-secosterols analyzed by click assay showed that modified proteins are only detected in the HMW region, indicating that secosterol adduction generates species highly prone to aggregate. Of note, SOD1-secosterol adducts containing up to five secosterol molecules were confirmed by MALDI-TOF analysis. Interestingly, mass spectrometry sequencing of SOD1 aggregates revealed preferential secosterol adduction to Lys residues located at the electrostatic loop (Lys 122, 128 and 136) and nearby the dimer interface (Lys 3 and 9). Altogether, our results show that secosterol aldehydes are increased in plasma of symptomatic ALS rats and represent a class of aldehydes that can potentially modify SOD1 enhancing its propensity to aggregate.

Original languageEnglish (US)
Pages (from-to)105-115
Number of pages11
JournalRedox Biology
Volume19
DOIs
StatePublished - Oct 1 2018

Fingerprint

Amyotrophic Lateral Sclerosis
Aldehydes
Agglomeration
Cholesterol
Rats
Plasmas
Neurodegenerative Diseases
Molecular Weight
Molecular weight
Neurodegenerative diseases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Motor Cortex
Motor Neurons
Pathology
Static Electricity
Dimers
Neurons
Mass spectrometry
Disease Progression
Electrostatics

Keywords

  • Amyotrophic Lateral Sclerosis
  • Neurodegenerative diseases
  • Protein aggregation
  • Secosterol aldehydes
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

Cite this

Cholesterol secosterol aldehyde adduction and aggregation of Cu,Zn-superoxide dismutase : Potential implications in ALS. / Dantas, Lucas S.; Chaves-Filho, Adriano B.; Coelho, Fernando R.; Cardoso Genaro De Mattos, Thiago; Tallman, Keri A.; Porter, Ned A.; Augusto, Ohara; Miyamoto, Sayuri.

In: Redox Biology, Vol. 19, 01.10.2018, p. 105-115.

Research output: Contribution to journalArticle

Dantas, Lucas S. ; Chaves-Filho, Adriano B. ; Coelho, Fernando R. ; Cardoso Genaro De Mattos, Thiago ; Tallman, Keri A. ; Porter, Ned A. ; Augusto, Ohara ; Miyamoto, Sayuri. / Cholesterol secosterol aldehyde adduction and aggregation of Cu,Zn-superoxide dismutase : Potential implications in ALS. In: Redox Biology. 2018 ; Vol. 19. pp. 105-115.
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abstract = "Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by degeneration of upper and lower motor neurons. While the fundamental causes of the disease are still unclear, the accumulation of Cu,Zn-superoxide dismutase (SOD1) immunoreactive aggregates is associated with familial ALS cases. Cholesterol 5,6-secosterol aldehydes (Seco A and Seco B) are reported to contribute to neurodegenerative disease pathology by inducing protein modification and aggregation. Here we have investigated the presence of secosterol aldehydes in ALS SOD1-G93A rats and their capacity to induce SOD1 aggregation. Secosterol aldehydes were analyzed in blood plasma, spinal cord and motor cortex of ALS rats at the pre-symptomatic and symptomatic stages. Seco B was significantly increased in plasma of symptomatic ALS rats compared to pre-symptomatic animals, suggesting an association with disease progression. In vitro experiments showed that both Seco A and Seco B induce the formation of high molecular weight (HMW) SOD1 aggregates with amorphous morphology. SOD1 adduction to ω-alkynyl-secosterols analyzed by click assay showed that modified proteins are only detected in the HMW region, indicating that secosterol adduction generates species highly prone to aggregate. Of note, SOD1-secosterol adducts containing up to five secosterol molecules were confirmed by MALDI-TOF analysis. Interestingly, mass spectrometry sequencing of SOD1 aggregates revealed preferential secosterol adduction to Lys residues located at the electrostatic loop (Lys 122, 128 and 136) and nearby the dimer interface (Lys 3 and 9). Altogether, our results show that secosterol aldehydes are increased in plasma of symptomatic ALS rats and represent a class of aldehydes that can potentially modify SOD1 enhancing its propensity to aggregate.",
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AU - Chaves-Filho, Adriano B.

AU - Coelho, Fernando R.

AU - Cardoso Genaro De Mattos, Thiago

AU - Tallman, Keri A.

AU - Porter, Ned A.

AU - Augusto, Ohara

AU - Miyamoto, Sayuri

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AB - Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by degeneration of upper and lower motor neurons. While the fundamental causes of the disease are still unclear, the accumulation of Cu,Zn-superoxide dismutase (SOD1) immunoreactive aggregates is associated with familial ALS cases. Cholesterol 5,6-secosterol aldehydes (Seco A and Seco B) are reported to contribute to neurodegenerative disease pathology by inducing protein modification and aggregation. Here we have investigated the presence of secosterol aldehydes in ALS SOD1-G93A rats and their capacity to induce SOD1 aggregation. Secosterol aldehydes were analyzed in blood plasma, spinal cord and motor cortex of ALS rats at the pre-symptomatic and symptomatic stages. Seco B was significantly increased in plasma of symptomatic ALS rats compared to pre-symptomatic animals, suggesting an association with disease progression. In vitro experiments showed that both Seco A and Seco B induce the formation of high molecular weight (HMW) SOD1 aggregates with amorphous morphology. SOD1 adduction to ω-alkynyl-secosterols analyzed by click assay showed that modified proteins are only detected in the HMW region, indicating that secosterol adduction generates species highly prone to aggregate. Of note, SOD1-secosterol adducts containing up to five secosterol molecules were confirmed by MALDI-TOF analysis. Interestingly, mass spectrometry sequencing of SOD1 aggregates revealed preferential secosterol adduction to Lys residues located at the electrostatic loop (Lys 122, 128 and 136) and nearby the dimer interface (Lys 3 and 9). Altogether, our results show that secosterol aldehydes are increased in plasma of symptomatic ALS rats and represent a class of aldehydes that can potentially modify SOD1 enhancing its propensity to aggregate.

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