Chlorovirus PBCV-1 encodes an active copper-zinc superoxide dismutase

Ming Kang, Garry A. Duncan, Charles Kuszynski, George Oyler, Jiayin Zheng, Donald F Becker, James L Van Etten

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Superoxide dismutases (SODs) are metalloproteins that protect organisms from toxic reactive oxygen species by catalyzing the conversion of superoxide anion to hydrogen peroxide and molecular oxygen. Chlorovirus PBCV-1 encodes a 187-amino-acid protein that resembles a Cu-Zn SOD with all of the conserved amino acid residues for binding copper and zinc (named cvSOD). cvSOD has an internal Met that results in a 165-amino-acid protein (named tcvSOD). Both cvSOD and tcvSOD recombinant proteins inhibited nitroblue tetrazolium reduction of superoxide anion generated in a xanthine-xanthine oxidase system in solution. tcvSOD was chosen for further characterization because it was easier to produce. Recombinant tcvSOD also inhibited a riboflavin photochemical reduction system in a polyacrylamide gel assay, which was blocked by the Cu-Zn SOD inhibitor cyanide but not by azide, which inhibits Fe and Mn SODs. A kcat/Km value for cvSOD was determined by stop-flow spectrophotometry as 1.28×108M-1 s-1, suggesting that cvSOD-catalyzed O2- dismutation was not a diffusion controlled encounter. The cvsod gene was expressed as a late gene, and cvSOD activity was detected in purified virions. Superoxide accumulated rapidly during virus infection, and circumstantial evidence indicates that cvSOD aids its decomposition to benefit virus replication. Cu-Zn SOD homologs have been described to occur in 3 other families of large DNA viruses, poxviruses, baculoviruses, and mimiviruses, which group as a clade. Interestingly, cvSOD does not group in the same clade as the other virus SODs but instead groups in an expanded clade that includes Cu-Zn SODs from many cellular organisms.

Original languageEnglish (US)
Pages (from-to)12541-12550
Number of pages10
JournalJournal of virology
Volume88
Issue number21
DOIs
StatePublished - Jan 1 2014

Fingerprint

Chlorovirus
Paramecium bursaria Chlorella virus 1
Superoxide Dismutase
Zinc
Copper
superoxide dismutase
copper
zinc
Superoxides
Amino Acids
Mimiviridae
superoxide anion
Metalloproteins
Poxviridae
Nitroblue Tetrazolium
Xanthine
DNA Viruses
Azides
Riboflavin
Xanthine Oxidase

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Chlorovirus PBCV-1 encodes an active copper-zinc superoxide dismutase. / Kang, Ming; Duncan, Garry A.; Kuszynski, Charles; Oyler, George; Zheng, Jiayin; Becker, Donald F; Van Etten, James L.

In: Journal of virology, Vol. 88, No. 21, 01.01.2014, p. 12541-12550.

Research output: Contribution to journalArticle

Kang M, Duncan GA, Kuszynski C, Oyler G, Zheng J, Becker DF et al. Chlorovirus PBCV-1 encodes an active copper-zinc superoxide dismutase. Journal of virology. 2014 Jan 1;88(21):12541-12550. https://doi.org/10.1128/JVI.02031-14
Kang, Ming ; Duncan, Garry A. ; Kuszynski, Charles ; Oyler, George ; Zheng, Jiayin ; Becker, Donald F ; Van Etten, James L. / Chlorovirus PBCV-1 encodes an active copper-zinc superoxide dismutase. In: Journal of virology. 2014 ; Vol. 88, No. 21. pp. 12541-12550.
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