Chloride-bicarbonate exchangers in the human fetal pancreas

Karen Hyde, Deborah Harrison, Michael A Hollingsworth, Ann Harris

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes a small conductance cAMP-activated chloride ion channel. In the CF pancreatic duct, mutations in CFTR cause a reduction in bicarbonate secretion. This is thought to result from CFTR operating in parallel with a chloride-bicarbonate (Cl-/HCO3-) exchanger, located in the apical membrane of pancreatic duct cells. The molecular basis of this Cl-/HCO3- exchanger has not been identified. A combination of screening cDNA libraries, RNase protection, and 5' RACE analysis was used to identify Cl-/HCO3- exchangers in human fetal pancreas. An AE2 Cl-/HCO3- exchanger was shown to be expressed in human fetal pancreas from the midtrimester of gestation, at a time when CF-associated pathology commences. In addition, an AE1 Cl-/HCO3- was identified in fetal pancreas but was absent from the adult pancreas and cultured ductal epithelial cells from fetal and adult pancreas.

Original languageEnglish (US)
Pages (from-to)315-321
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume263
Issue number2
DOIs
StatePublished - Sep 24 1999

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Chloride-Bicarbonate Antiporters
Cystic Fibrosis Transmembrane Conductance Regulator
Pancreas
Cystic Fibrosis
Bicarbonates
Ducts
Pancreatic Ducts
Chloride Channels
Pathology
Ribonucleases
Gene Library
Mutation
Chlorides
Second Pregnancy Trimester
Regulator Genes
Screening
Genes
Membranes
Epithelial Cells
Pregnancy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chloride-bicarbonate exchangers in the human fetal pancreas. / Hyde, Karen; Harrison, Deborah; Hollingsworth, Michael A; Harris, Ann.

In: Biochemical and Biophysical Research Communications, Vol. 263, No. 2, 24.09.1999, p. 315-321.

Research output: Contribution to journalArticle

Hyde, Karen ; Harrison, Deborah ; Hollingsworth, Michael A ; Harris, Ann. / Chloride-bicarbonate exchangers in the human fetal pancreas. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 263, No. 2. pp. 315-321.
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