Chlorella viruses prevent multiple infections by depolarizing the host membrane

Timo Greiner, Florian Frohns, Ming Kang, James L Van Etten, Anja Käsmann, Anna Moroni, Brigitte Hertel, Gerhard Thiel

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Previous experiments established that when the unicellular green alga Chlorella NC64A is inoculated with two viruses, usually only one virus replicates in a single cell. That is, the viruses mutually exclude one another. In the current study, we explore the possibility that virus-induced host membrane depolarization, at least partially caused by a virus-encoded K+ channel (Kcv), is involved in this mutual exclusion. Two chlorella viruses, PBCV-1 and NY-2A, were chosen for the study because (i) they can be distinguished by real-time PCR and (ii) they exhibit differential sensitivity to Cs+, a well-known K+ channel blocker. PBCV-1-induced host membrane depolarization, Kcv channel activity and plaque formation are only slightly affected by Cs+, whereas all three NY-2A-induced events are strongly inhibited by Cs+. The addition of one virus 5-15 min before the other results primarily in replication of the first virus. However, if virus NY-2A-induced membrane depolarization of the host is blocked by Cs+, PBCV-1 is not excluded. We conclude that virus-induced membrane depolarization is at least partially responsible for the exclusion phenomenon.

Original languageEnglish (US)
Pages (from-to)2033-2039
Number of pages7
JournalJournal of General Virology
Volume90
Issue number8
DOIs
StatePublished - Oct 8 2009

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Chlorella
Viruses
Membranes
Infection
Chlorophyta
Virus Replication
Real-Time Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology

Cite this

Chlorella viruses prevent multiple infections by depolarizing the host membrane. / Greiner, Timo; Frohns, Florian; Kang, Ming; Van Etten, James L; Käsmann, Anja; Moroni, Anna; Hertel, Brigitte; Thiel, Gerhard.

In: Journal of General Virology, Vol. 90, No. 8, 08.10.2009, p. 2033-2039.

Research output: Contribution to journalArticle

Greiner, T, Frohns, F, Kang, M, Van Etten, JL, Käsmann, A, Moroni, A, Hertel, B & Thiel, G 2009, 'Chlorella viruses prevent multiple infections by depolarizing the host membrane', Journal of General Virology, vol. 90, no. 8, pp. 2033-2039. https://doi.org/10.1099/vir.0.010629-0
Greiner, Timo ; Frohns, Florian ; Kang, Ming ; Van Etten, James L ; Käsmann, Anja ; Moroni, Anna ; Hertel, Brigitte ; Thiel, Gerhard. / Chlorella viruses prevent multiple infections by depolarizing the host membrane. In: Journal of General Virology. 2009 ; Vol. 90, No. 8. pp. 2033-2039.
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