Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection

Monika Neupärtl, Christine Meyer, Isabell Woll, Florian Frohns, Ming Kang, James L. Van Etten, Detlef Kramer, Brigitte Hertel, Anna Moroni, Gerhard Thiel

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K+ channel (Kcv) into the host membrane. To examine the effect of an elevated conductance, we monitored the virus-stimulated efflux of K+ from the chlorella cells. The results indicate that all 8 chlorella viruses tested evoked a host specific K+ efflux with a concomitant decrease in the intracellular K+. This K+ efflux is partially reduced by blockers of the Kcv channel. Qualitatively these results support the hypothesis that depolarization and K+ efflux are at least partially mediated by Kcv. The virus-triggered K+ efflux occurs in the same time frame as host cell wall degradation and ejection of viral DNA. Therefore, it is reasonable to postulate that loss of K+ and associated water fluxes from the host lower the pressure barrier to aid ejection of DNA from the virus particles into the host.

Original languageEnglish (US)
Pages (from-to)340-348
Number of pages9
JournalVirology
Volume372
Issue number2
DOIs
StatePublished - Mar 15 2008

Fingerprint

Chlorella
Viruses
Infection
DNA Viruses
Viral DNA
Virion
Cell Wall
Pressure
Membranes
Water

Keywords

  • Chlorella viruses
  • K efflux
  • PBCV-1
  • Phycodnaviridae
  • Viral K channel Kcv

ASJC Scopus subject areas

  • Virology

Cite this

Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection. / Neupärtl, Monika; Meyer, Christine; Woll, Isabell; Frohns, Florian; Kang, Ming; Van Etten, James L.; Kramer, Detlef; Hertel, Brigitte; Moroni, Anna; Thiel, Gerhard.

In: Virology, Vol. 372, No. 2, 15.03.2008, p. 340-348.

Research output: Contribution to journalArticle

Neupärtl, M, Meyer, C, Woll, I, Frohns, F, Kang, M, Van Etten, JL, Kramer, D, Hertel, B, Moroni, A & Thiel, G 2008, 'Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection', Virology, vol. 372, no. 2, pp. 340-348. https://doi.org/10.1016/j.virol.2007.10.024
Neupärtl, Monika ; Meyer, Christine ; Woll, Isabell ; Frohns, Florian ; Kang, Ming ; Van Etten, James L. ; Kramer, Detlef ; Hertel, Brigitte ; Moroni, Anna ; Thiel, Gerhard. / Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection. In: Virology. 2008 ; Vol. 372, No. 2. pp. 340-348.
@article{b27d632bb05d43f6a77aba3b92a8f776,
title = "Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection",
abstract = "Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K+ channel (Kcv) into the host membrane. To examine the effect of an elevated conductance, we monitored the virus-stimulated efflux of K+ from the chlorella cells. The results indicate that all 8 chlorella viruses tested evoked a host specific K+ efflux with a concomitant decrease in the intracellular K+. This K+ efflux is partially reduced by blockers of the Kcv channel. Qualitatively these results support the hypothesis that depolarization and K+ efflux are at least partially mediated by Kcv. The virus-triggered K+ efflux occurs in the same time frame as host cell wall degradation and ejection of viral DNA. Therefore, it is reasonable to postulate that loss of K+ and associated water fluxes from the host lower the pressure barrier to aid ejection of DNA from the virus particles into the host.",
keywords = "Chlorella viruses, K efflux, PBCV-1, Phycodnaviridae, Viral K channel Kcv",
author = "Monika Neup{\"a}rtl and Christine Meyer and Isabell Woll and Florian Frohns and Ming Kang and {Van Etten}, {James L.} and Detlef Kramer and Brigitte Hertel and Anna Moroni and Gerhard Thiel",
year = "2008",
month = "3",
day = "15",
doi = "10.1016/j.virol.2007.10.024",
language = "English (US)",
volume = "372",
pages = "340--348",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection

AU - Neupärtl, Monika

AU - Meyer, Christine

AU - Woll, Isabell

AU - Frohns, Florian

AU - Kang, Ming

AU - Van Etten, James L.

AU - Kramer, Detlef

AU - Hertel, Brigitte

AU - Moroni, Anna

AU - Thiel, Gerhard

PY - 2008/3/15

Y1 - 2008/3/15

N2 - Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K+ channel (Kcv) into the host membrane. To examine the effect of an elevated conductance, we monitored the virus-stimulated efflux of K+ from the chlorella cells. The results indicate that all 8 chlorella viruses tested evoked a host specific K+ efflux with a concomitant decrease in the intracellular K+. This K+ efflux is partially reduced by blockers of the Kcv channel. Qualitatively these results support the hypothesis that depolarization and K+ efflux are at least partially mediated by Kcv. The virus-triggered K+ efflux occurs in the same time frame as host cell wall degradation and ejection of viral DNA. Therefore, it is reasonable to postulate that loss of K+ and associated water fluxes from the host lower the pressure barrier to aid ejection of DNA from the virus particles into the host.

AB - Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K+ channel (Kcv) into the host membrane. To examine the effect of an elevated conductance, we monitored the virus-stimulated efflux of K+ from the chlorella cells. The results indicate that all 8 chlorella viruses tested evoked a host specific K+ efflux with a concomitant decrease in the intracellular K+. This K+ efflux is partially reduced by blockers of the Kcv channel. Qualitatively these results support the hypothesis that depolarization and K+ efflux are at least partially mediated by Kcv. The virus-triggered K+ efflux occurs in the same time frame as host cell wall degradation and ejection of viral DNA. Therefore, it is reasonable to postulate that loss of K+ and associated water fluxes from the host lower the pressure barrier to aid ejection of DNA from the virus particles into the host.

KW - Chlorella viruses

KW - K efflux

KW - PBCV-1

KW - Phycodnaviridae

KW - Viral K channel Kcv

UR - http://www.scopus.com/inward/record.url?scp=39649096556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39649096556&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2007.10.024

DO - 10.1016/j.virol.2007.10.024

M3 - Article

C2 - 18045641

AN - SCOPUS:39649096556

VL - 372

SP - 340

EP - 348

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -