Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends

Jan Rohozinski, Lois E. Girton, James L. Van Etten

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Abstract

Pulsed field electrophoresis established that Chlorella viruses contain linear, nonpermuted, 330- to 380-kb dsDNA genomes. Terminal DNA restriction fragments of one virus, PBCV-1, were identified by Bal31 exonuclease digestion; the termini probably contain covalently closed hairpin ends. The end fragments cross-hybridize indicating terminal repetition; the region of repetition extends no more than 2.5 kb from the ends.

Original languageEnglish (US)
Pages (from-to)363-369
Number of pages7
JournalVirology
Volume168
Issue number2
DOIs
Publication statusPublished - Feb 1989

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ASJC Scopus subject areas

  • Virology

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