Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends

Jan Rohozinski, Lois E. Girton, James L. Van Etten

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Pulsed field electrophoresis established that Chlorella viruses contain linear, nonpermuted, 330- to 380-kb dsDNA genomes. Terminal DNA restriction fragments of one virus, PBCV-1, were identified by Bal31 exonuclease digestion; the termini probably contain covalently closed hairpin ends. The end fragments cross-hybridize indicating terminal repetition; the region of repetition extends no more than 2.5 kb from the ends.

Original languageEnglish (US)
Pages (from-to)363-369
Number of pages7
JournalVirology
Volume168
Issue number2
DOIs
StatePublished - Feb 1989

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Chlorella
Genome
Viruses
Exonucleases
DNA
Electrophoresis
Digestion

ASJC Scopus subject areas

  • Virology

Cite this

Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends. / Rohozinski, Jan; Girton, Lois E.; Van Etten, James L.

In: Virology, Vol. 168, No. 2, 02.1989, p. 363-369.

Research output: Contribution to journalArticle

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