Chlorella virus SC-1A encodes at least five functional and one nonfunctional DNA methyltransferases

Qiudeng Que, Yanping Zhang, Michael Nelson, Susan Ropp, Dwight E. Burbank, James L Van Etten

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N6-methyldeoxyadenine (m6A) MTases, M·CviSI (TGCmA), M·CviSII (CmATG), M·CviSIII (TCGmA) and M·CviSIV (GmATC), one 5-methyldeoxycytosine (m5C) MTase, M·CviSV (~ RCmCG), and one nonfunctional m5C MTase, M·CviSVI, which is homologous to the MTase M·CviJI [RGmC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m6A MTases (M·CviSI, M·CviSII, and M·CviSIII) and the nonfunctional m5C MTase were cloned and sequenced. Neither M·CviSI nor M·CviSIII genes hybridized to genes for their respective isomethylomers, M·CviRI and M·CviBIII, from other chlorella viruses. However, the M·CviSII gene hybridized strongly to its M·CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m5C MTase. The three cloned m6A MTase genes are distributed throughout the approx. 345 kb SC-1A genome.

Original languageEnglish (US)
Pages (from-to)237-244
Number of pages8
JournalGene
Volume190
Issue number2
DOIs
StatePublished - Jun 7 1997

Fingerprint

Chlorella
Methyltransferases
Viruses
DNA
Genes
Genome
Terminal Repeat Sequences

Keywords

  • 5-Methyldeoxycytosine methyltransferase
  • 5-Methyldeoxycytosine methyltransferase pseudogene
  • N-methyldeoxyadenine methyltransferase
  • Recombinant DNA

ASJC Scopus subject areas

  • Medicine(all)
  • Genetics

Cite this

Chlorella virus SC-1A encodes at least five functional and one nonfunctional DNA methyltransferases. / Que, Qiudeng; Zhang, Yanping; Nelson, Michael; Ropp, Susan; Burbank, Dwight E.; Van Etten, James L.

In: Gene, Vol. 190, No. 2, 07.06.1997, p. 237-244.

Research output: Contribution to journalArticle

Que, Qiudeng ; Zhang, Yanping ; Nelson, Michael ; Ropp, Susan ; Burbank, Dwight E. ; Van Etten, James L. / Chlorella virus SC-1A encodes at least five functional and one nonfunctional DNA methyltransferases. In: Gene. 1997 ; Vol. 190, No. 2. pp. 237-244.
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abstract = "Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N6-methyldeoxyadenine (m6A) MTases, M·CviSI (TGCmA), M·CviSII (CmATG), M·CviSIII (TCGmA) and M·CviSIV (GmATC), one 5-methyldeoxycytosine (m5C) MTase, M·CviSV (~ RCmCG), and one nonfunctional m5C MTase, M·CviSVI, which is homologous to the MTase M·CviJI [RGmC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m6A MTases (M·CviSI, M·CviSII, and M·CviSIII) and the nonfunctional m5C MTase were cloned and sequenced. Neither M·CviSI nor M·CviSIII genes hybridized to genes for their respective isomethylomers, M·CviRI and M·CviBIII, from other chlorella viruses. However, the M·CviSII gene hybridized strongly to its M·CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m5C MTase. The three cloned m6A MTase genes are distributed throughout the approx. 345 kb SC-1A genome.",
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