Chlorella virus PBCV-1 encodes functional glutamine

Fructose-6- phosphate amidotransferase and UDP-glucose dehydrogenase enzymes

Dorit Landstein, Michael V. Graves, Dwight E. Burbank, Paul Deangelis, James L Van Etten

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

DNA sequence analysis of the 330-kb Chlorella virus PBCV-1 genome unexpectedly revealed several open reading frames which encode proteins that are homologous to sugar-manipulating enzymes including glutamine:fructose-6- phosphate amidotransferase (GFAT), UDP-glucose dehydrogenase (UDP-GlcDH), and hyaluronan synthase (HAS). PBCV-1 genes encoding the putative GFAT and UDP- GlcDH enzymes were expressed in Escherichia coli and both recombinant proteins have the predicted enzyme activity in cell free extracts. These same two genes are transcribed early in PBCV-1 infection, and both genes are widespread among the Chlorella viruses. The products of the reactions catalyzed by these two enzymes are precursors in the biosynthesis of hyaluronan polysaccharide. Previous experiments established that, like the GFAT and UDP-GlcDH genes, the HAS gene is transcribed early and encodes a functional enzyme (DeAngelis, P.L., Jing. W., Graves, M.V., Burbank, D.E., and Van Etten, J.L. (1997) Science 278, 1800-1803). Interestingly, the predicted amino-acid sequences of the PBCV-1 GFAT and UDP-GlcDH enzymes are more similar to bacterial GFAT and UDP-GlcDH enzymes than to their eukaryotic counterparts. In contrast, the amino-acid sequence of the PBCV-1 HAS enzyme more closely resembles eukaryotic enzymes.

Original languageEnglish (US)
Pages (from-to)388-396
Number of pages9
JournalVirology
Volume250
Issue number2
DOIs
StatePublished - Oct 25 1998

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Uridine Diphosphate Glucose Dehydrogenase
Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
Chlorella
Viruses
Enzymes
Genes
Amino Acid Sequence
Enzyme Precursors
Uridine Diphosphate
Hyaluronic Acid
Cell Extracts
DNA Sequence Analysis
Recombinant Proteins
Open Reading Frames
Polysaccharides
Genome
Escherichia coli

ASJC Scopus subject areas

  • Virology

Cite this

Chlorella virus PBCV-1 encodes functional glutamine : Fructose-6- phosphate amidotransferase and UDP-glucose dehydrogenase enzymes. / Landstein, Dorit; Graves, Michael V.; Burbank, Dwight E.; Deangelis, Paul; Van Etten, James L.

In: Virology, Vol. 250, No. 2, 25.10.1998, p. 388-396.

Research output: Contribution to journalArticle

Landstein, Dorit ; Graves, Michael V. ; Burbank, Dwight E. ; Deangelis, Paul ; Van Etten, James L. / Chlorella virus PBCV-1 encodes functional glutamine : Fructose-6- phosphate amidotransferase and UDP-glucose dehydrogenase enzymes. In: Virology. 1998 ; Vol. 250, No. 2. pp. 388-396.
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