Chlorella virus PBCV-1 encodes a functional homospermidine synthase

Annette Kaiser, Michael Vollmert, Dorothea Tholl, Michael V. Graves, James R. Gurnon, Weimei Xing, Amber D. Lisec, Kenneth Nickerson, James L Van Etten

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Sequence analysis of the 330-kb genome of chlorella virus Paramecium bursaria chlorella virus 1 (PBCV-1) revealed an open reading frame, A237R, that encodes a protein with 34% amino acid identity to homospermidine synthase from Rhodopseudomonas viridis. Expression of the a237r gene product in Escherichia coli established that the recombinant enzyme catalyzes the NAD+-dependent formation of homospermidine from two molecules of putrescine. The a237r gene is expressed late in PBCV-1 infection. Both uninfected and PBCV-1-infected chlorella, as well as PBCV-1 virions, contain homospermidine, along with the more common polyamines putrescine, spermidine, and cadaverine. The total number of polyamine molecules per virion (~539) is too small to significantly neutralize the virus double-stranded DNA (>660,000 nucleotides). Consequently, the biological significance of the homospermidine synthase gene is unknown. However, the gene is widespread among the chlorella viruses. To our knowledge, this is the first report of a virus encoding an enzyme involved in polyamine biosynthesis.

Original languageEnglish (US)
Pages (from-to)254-262
Number of pages9
JournalVirology
Volume263
Issue number1
DOIs
StatePublished - Oct 10 1999

Fingerprint

Paramecium
Chlorella
Viruses
Polyamines
Putrescine
Virion
Rhodopseudomonas
Cadaverine
Genes
Spermidine
Virus Diseases
Enzymes
homospermidine synthetase
NAD
Open Reading Frames
Sequence Analysis
Nucleotides
Genome
Escherichia coli
Gene Expression

Keywords

  • Chlorella viruses
  • DsDNA virus
  • Homospermidine
  • Homospermidine synthase
  • Ornithine decarboxylase
  • PBCV-1
  • Phycodnaviridae
  • Polyamines

ASJC Scopus subject areas

  • Virology

Cite this

Kaiser, A., Vollmert, M., Tholl, D., Graves, M. V., Gurnon, J. R., Xing, W., ... Van Etten, J. L. (1999). Chlorella virus PBCV-1 encodes a functional homospermidine synthase. Virology, 263(1), 254-262. https://doi.org/10.1006/viro.1999.9972

Chlorella virus PBCV-1 encodes a functional homospermidine synthase. / Kaiser, Annette; Vollmert, Michael; Tholl, Dorothea; Graves, Michael V.; Gurnon, James R.; Xing, Weimei; Lisec, Amber D.; Nickerson, Kenneth; Van Etten, James L.

In: Virology, Vol. 263, No. 1, 10.10.1999, p. 254-262.

Research output: Contribution to journalArticle

Kaiser, A, Vollmert, M, Tholl, D, Graves, MV, Gurnon, JR, Xing, W, Lisec, AD, Nickerson, K & Van Etten, JL 1999, 'Chlorella virus PBCV-1 encodes a functional homospermidine synthase', Virology, vol. 263, no. 1, pp. 254-262. https://doi.org/10.1006/viro.1999.9972
Kaiser A, Vollmert M, Tholl D, Graves MV, Gurnon JR, Xing W et al. Chlorella virus PBCV-1 encodes a functional homospermidine synthase. Virology. 1999 Oct 10;263(1):254-262. https://doi.org/10.1006/viro.1999.9972
Kaiser, Annette ; Vollmert, Michael ; Tholl, Dorothea ; Graves, Michael V. ; Gurnon, James R. ; Xing, Weimei ; Lisec, Amber D. ; Nickerson, Kenneth ; Van Etten, James L. / Chlorella virus PBCV-1 encodes a functional homospermidine synthase. In: Virology. 1999 ; Vol. 263, No. 1. pp. 254-262.
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