Chlorella virus NY-2A encodes at least 12 DNA endonuclease/methyltransferase genes

Yanping Zhang, Michael Nelson, Joe Nietfeldt, Yuannan Xia, Dwight Burbank, Susan Ropp, James L. Van Etten

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6- methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA methyltransferases: 7 6mA-specific methyltransferases, M.CviQI (GT(m)AC), M.CvQII (R(m)AR), M.CviQIII (TCG(m)A), M.CviQIV (G(m)ATC), M.CviQV (TGC(m)A), M.CviQVI (G(m)ANTC), and M.CviQVII (C(m)ATG); and 3 5mC-specific methyltransferases, M.CviQVIII [RG(m)C(T/C/G)], M.CviQIX ((m)CC), and M.CviQX ((m)CGR). Five of the 6mA methyltransferase genes, M.CviQI, M. CviQIII, M. CviQV, M.CviQVI, and M.CviQVII, were cloned and sequenced. In addition, 2 site-specific endonuclease activities, R.CviQI (G/TAC) and NY2A-nickase (R/AG), were detected in cell-free extracts from NY- 2A virus-infected chlorella. Therefore, the NY-2A genome contains at least 12 DNA methyltransferase and endonuclease genes which, altogether, compose about 3-4% of the virus genome.

Original languageEnglish (US)
Pages (from-to)366-375
Number of pages10
JournalVirology
Volume240
Issue number2
DOIs
StatePublished - Jan 15 1998

Fingerprint

Chlorella
Deoxyribonuclease I
Methyltransferases
Viruses
5-Methylcytosine
Genes
Genome
Cytosine
DNA
DNA Restriction Enzymes
Adenine
Cell Extracts
Methylation

ASJC Scopus subject areas

  • Virology

Cite this

Chlorella virus NY-2A encodes at least 12 DNA endonuclease/methyltransferase genes. / Zhang, Yanping; Nelson, Michael; Nietfeldt, Joe; Xia, Yuannan; Burbank, Dwight; Ropp, Susan; Van Etten, James L.

In: Virology, Vol. 240, No. 2, 15.01.1998, p. 366-375.

Research output: Contribution to journalArticle

Zhang, Y, Nelson, M, Nietfeldt, J, Xia, Y, Burbank, D, Ropp, S & Van Etten, JL 1998, 'Chlorella virus NY-2A encodes at least 12 DNA endonuclease/methyltransferase genes', Virology, vol. 240, no. 2, pp. 366-375. https://doi.org/10.1006/viro.1997.8936
Zhang Y, Nelson M, Nietfeldt J, Xia Y, Burbank D, Ropp S et al. Chlorella virus NY-2A encodes at least 12 DNA endonuclease/methyltransferase genes. Virology. 1998 Jan 15;240(2):366-375. https://doi.org/10.1006/viro.1997.8936
Zhang, Yanping ; Nelson, Michael ; Nietfeldt, Joe ; Xia, Yuannan ; Burbank, Dwight ; Ropp, Susan ; Van Etten, James L. / Chlorella virus NY-2A encodes at least 12 DNA endonuclease/methyltransferase genes. In: Virology. 1998 ; Vol. 240, No. 2. pp. 366-375.
@article{47b3c6556e0a415595bbccc223baaed8,
title = "Chlorella virus NY-2A encodes at least 12 DNA endonuclease/methyltransferase genes",
abstract = "The 380-kb chlorella virus NY-2A genome is highly methylated; 45{\%} of the cytosines are 5-methylcytosine (5mC) and 37{\%} of the adenines are N6- methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA methyltransferases: 7 6mA-specific methyltransferases, M.CviQI (GT(m)AC), M.CvQII (R(m)AR), M.CviQIII (TCG(m)A), M.CviQIV (G(m)ATC), M.CviQV (TGC(m)A), M.CviQVI (G(m)ANTC), and M.CviQVII (C(m)ATG); and 3 5mC-specific methyltransferases, M.CviQVIII [RG(m)C(T/C/G)], M.CviQIX ((m)CC), and M.CviQX ((m)CGR). Five of the 6mA methyltransferase genes, M.CviQI, M. CviQIII, M. CviQV, M.CviQVI, and M.CviQVII, were cloned and sequenced. In addition, 2 site-specific endonuclease activities, R.CviQI (G/TAC) and NY2A-nickase (R/AG), were detected in cell-free extracts from NY- 2A virus-infected chlorella. Therefore, the NY-2A genome contains at least 12 DNA methyltransferase and endonuclease genes which, altogether, compose about 3-4{\%} of the virus genome.",
author = "Yanping Zhang and Michael Nelson and Joe Nietfeldt and Yuannan Xia and Dwight Burbank and Susan Ropp and {Van Etten}, {James L.}",
year = "1998",
month = "1",
day = "15",
doi = "10.1006/viro.1997.8936",
language = "English (US)",
volume = "240",
pages = "366--375",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Chlorella virus NY-2A encodes at least 12 DNA endonuclease/methyltransferase genes

AU - Zhang, Yanping

AU - Nelson, Michael

AU - Nietfeldt, Joe

AU - Xia, Yuannan

AU - Burbank, Dwight

AU - Ropp, Susan

AU - Van Etten, James L.

PY - 1998/1/15

Y1 - 1998/1/15

N2 - The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6- methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA methyltransferases: 7 6mA-specific methyltransferases, M.CviQI (GT(m)AC), M.CvQII (R(m)AR), M.CviQIII (TCG(m)A), M.CviQIV (G(m)ATC), M.CviQV (TGC(m)A), M.CviQVI (G(m)ANTC), and M.CviQVII (C(m)ATG); and 3 5mC-specific methyltransferases, M.CviQVIII [RG(m)C(T/C/G)], M.CviQIX ((m)CC), and M.CviQX ((m)CGR). Five of the 6mA methyltransferase genes, M.CviQI, M. CviQIII, M. CviQV, M.CviQVI, and M.CviQVII, were cloned and sequenced. In addition, 2 site-specific endonuclease activities, R.CviQI (G/TAC) and NY2A-nickase (R/AG), were detected in cell-free extracts from NY- 2A virus-infected chlorella. Therefore, the NY-2A genome contains at least 12 DNA methyltransferase and endonuclease genes which, altogether, compose about 3-4% of the virus genome.

AB - The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6- methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA methyltransferases: 7 6mA-specific methyltransferases, M.CviQI (GT(m)AC), M.CvQII (R(m)AR), M.CviQIII (TCG(m)A), M.CviQIV (G(m)ATC), M.CviQV (TGC(m)A), M.CviQVI (G(m)ANTC), and M.CviQVII (C(m)ATG); and 3 5mC-specific methyltransferases, M.CviQVIII [RG(m)C(T/C/G)], M.CviQIX ((m)CC), and M.CviQX ((m)CGR). Five of the 6mA methyltransferase genes, M.CviQI, M. CviQIII, M. CviQV, M.CviQVI, and M.CviQVII, were cloned and sequenced. In addition, 2 site-specific endonuclease activities, R.CviQI (G/TAC) and NY2A-nickase (R/AG), were detected in cell-free extracts from NY- 2A virus-infected chlorella. Therefore, the NY-2A genome contains at least 12 DNA methyltransferase and endonuclease genes which, altogether, compose about 3-4% of the virus genome.

UR - http://www.scopus.com/inward/record.url?scp=0032518484&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032518484&partnerID=8YFLogxK

U2 - 10.1006/viro.1997.8936

DO - 10.1006/viro.1997.8936

M3 - Article

C2 - 9454710

AN - SCOPUS:0032518484

VL - 240

SP - 366

EP - 375

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -