Chlamydiazyme plus blocking assay to detect Chlamydia trachomatis in endocervical specimens

R. D. Mills, A. Young, K. Cain, T. M H Blair, Michael Allen Sitorius, G. L. Woods

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Three methods to detect Chlamydia trachomatis in endocervical swab specimens collected from 502 women with genitourinary or abdominopelvic symptoms were evaluated: (1) a direct immunofluorescence assay, (2) an enzyme-linked immunoabsorbent assay, confirming positive samples with a blocking assay, and (3) conventional tissue cell culture. C. trachomatis was detected by at least one method in 72 specimens, of which 56 (11%) were determined to be true-positive results by repeated testing and by performing a confirmatory assay. The sensitivity, specificity, and positive and negative predictive values were 91%, 100%, 100%, and 99%, respectively, for culture and the enzyme-linked immunoabsorbent assay plus blocking assay and 74%, 98%, 83%, and 96%, respectively, for the direct immunofluorescence assay. In this population of women, using the enzyme-linked immunoabsorbent assay with the confirmatory assay is a rapid, reliable, and cost-effective alternative to culture for diagnosing infection with C. trachomatis.

Original languageEnglish (US)
Pages (from-to)209-212
Number of pages4
JournalAmerican journal of clinical pathology
Volume97
Issue number2
DOIs
StatePublished - Jan 1 1992

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Chlamydia trachomatis
Direct Fluorescent Antibody Technique
Enzymes
Cell Culture Techniques
Costs and Cost Analysis
Sensitivity and Specificity
Infection
Population

Keywords

  • Blocking assay
  • Chlamydia trachomatis
  • Chlamydiazyme
  • Enzyme-linked immunoassay

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Chlamydiazyme plus blocking assay to detect Chlamydia trachomatis in endocervical specimens. / Mills, R. D.; Young, A.; Cain, K.; Blair, T. M H; Sitorius, Michael Allen; Woods, G. L.

In: American journal of clinical pathology, Vol. 97, No. 2, 01.01.1992, p. 209-212.

Research output: Contribution to journalArticle

Mills, R. D. ; Young, A. ; Cain, K. ; Blair, T. M H ; Sitorius, Michael Allen ; Woods, G. L. / Chlamydiazyme plus blocking assay to detect Chlamydia trachomatis in endocervical specimens. In: American journal of clinical pathology. 1992 ; Vol. 97, No. 2. pp. 209-212.
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