Chlamydia co-opts the rod shape-determining proteins MreB and Pbp2 for cell division

Scot P. Ouellette, Gouzel Karimova, Agathe Subtil, Daniel Ladant

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Chlamydiae are obligate intracellular bacterial pathogens that have extensively reduced their genome in adapting to the intracellular environment. The chlamydial genome contains only three annotated cell division genes and lacks ftsZ. How this obligate intracellular pathogen divides is uncharacterized. Chlamydiae contain two high-molecular-weight (HMW) penicillin binding proteins (Pbp) implicated in peptidoglycan synthesis, Pbp2 and Pbp3/FtsI. We show here, using HMW Pbp-specific penicillin derivatives, that both Pbp2 and Pbp3 are essential for chlamydial cell division. Ultrastructural analyses of antibiotic-treated cultures revealed distinct phenotypes: Pbp2 inhibition induced internal cell bodies within a single outer membrane whereas Pbp3 inhibition induced elongated phenotypes with little internal division. Each HMW Pbp interacts with the Chlamydia cell division protein FtsK. Chlamydiae are coccoid yet contain MreB, a rod shape-determining protein linked to Pbp2 in bacilli. Using MreB-specific antibiotics, we show that MreB is essential for chlamydial growth and division. Importantly, co-treatment with MreB-specific and Pbp-specific antibiotics resulted in the MreB-inhibited phenotype, placing MreB upstream of Pbp function in chlamydial cell division. Finally, we showed that MreB also interacts with FtsK. We propose that, in Chlamydia, MreB acts as a central co-ordinator at the division site to substitute for the lack of FtsZ in this bacterium.

Original languageEnglish (US)
Pages (from-to)164-178
Number of pages15
JournalMolecular Microbiology
Volume85
Issue number1
DOIs
StatePublished - Jul 1 2012

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Penicillin-Binding Proteins
Chlamydia
Cell Division
Molecular Weight
Anti-Bacterial Agents
Proteins
Phenotype
Genome
Peptidoglycan
Penicillins
Bacillus
Bacteria
Membranes
Growth
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Chlamydia co-opts the rod shape-determining proteins MreB and Pbp2 for cell division. / Ouellette, Scot P.; Karimova, Gouzel; Subtil, Agathe; Ladant, Daniel.

In: Molecular Microbiology, Vol. 85, No. 1, 01.07.2012, p. 164-178.

Research output: Contribution to journalArticle

Ouellette, Scot P. ; Karimova, Gouzel ; Subtil, Agathe ; Ladant, Daniel. / Chlamydia co-opts the rod shape-determining proteins MreB and Pbp2 for cell division. In: Molecular Microbiology. 2012 ; Vol. 85, No. 1. pp. 164-178.
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