Chemotype and aggressiveness of isolates of Fusarium graminearum causing head blight of wheat in Nebraska

Anita Panthi, Heather Hallen-Adams, Stephen N. Wegulo, John Hernandez Nopsa, P. Stephen Baenziger

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Fusarium head blight (FHB) or scab of wheat (Triticum aestivum L.) and its associated mycotoxin deoxynivalenol (DON) can cause devastating economic losses. In North America, FHB of wheat is caused mainly by Fusarium graminearum Schwabe [sexual stage Gibberella zeae (Schwein.) Petch]. In this study, the chemotype of 73 single-spore isolates of F. graminearum obtained from wheat kernels collected from 2007 to 2010 from FHB-Affected fields and grain elevators in Nebraska was determined to be 15-ADON. Eight selected isolates were evaluated for aggressiveness on spikes and DON production in grain using the FHB-susceptible spring wheat cultivar Wheaton. Aggressiveness was quantified as disease severity and area under the disease progress curve (AUDPC). Disease severity at 21 days after inoculation ranged from 40.9% to 99.5% and AUDPC ranged from 284%-days to 1279%-days. DON production in grain, measured by enzyme-linked immunosorbent assay (ELISA), ranged from 0.2 to 11.0 g g-1. Aggressiveness as measured using AUDPC was positively and linearly related to DON production in grain (R2 = 0.98; P < 0.0001). Isolates that produced the most DON were also the most aggressive. These results confirmed previous findings that the predominant F. graminearum chemotype in North America is 15-ADON and suggest that in Nebraska, there are highly aggressive populations of F. graminearum with high DON production capacity that have potential to cause severe losses due to reduction in yield and grain quality. This information will enhance farmers preparedness and willingness to apply management measures in years with a high risk for FHB outbreaks and will help researchers better understand FHB.

Original languageEnglish (US)
Pages (from-to)447-455
Number of pages9
JournalCanadian Journal of Plant Pathology
Volume36
Issue number4
DOIs
StatePublished - Oct 2 2014

Fingerprint

head blight
Fusarium head blight
chemotypes
Fusarium graminearum
deoxynivalenol
aggression
wheat
15-acetyldeoxynivalenol
disease severity
grain elevators
teleomorphs
spring wheat
mycotoxins
Triticum aestivum
inflorescences
spores
researchers
enzyme-linked immunosorbent assay
farmers
economics

Keywords

  • AUDPC disease severity
  • Triticum aestivum
  • deoxynivalenol
  • nivalenol
  • scab

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Chemotype and aggressiveness of isolates of Fusarium graminearum causing head blight of wheat in Nebraska. / Panthi, Anita; Hallen-Adams, Heather; Wegulo, Stephen N.; Hernandez Nopsa, John; Stephen Baenziger, P.

In: Canadian Journal of Plant Pathology, Vol. 36, No. 4, 02.10.2014, p. 447-455.

Research output: Contribution to journalArticle

Panthi, Anita ; Hallen-Adams, Heather ; Wegulo, Stephen N. ; Hernandez Nopsa, John ; Stephen Baenziger, P. / Chemotype and aggressiveness of isolates of Fusarium graminearum causing head blight of wheat in Nebraska. In: Canadian Journal of Plant Pathology. 2014 ; Vol. 36, No. 4. pp. 447-455.
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