Chemical shift assignments of the connexin45 carboxyl terminal domain

Monomer and dimer conformations

Jennifer L. Kopanic, Paul L Sorgen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Connexin45 (Cx45) is a gap junction protein involved in cell-to-cell communication in the heart and other tissues. Here we report the 1H, 15N, and 13C resonance assignments for the monomer and dimer conformations of the Cx45 carboxyl terminal (Cx45CT) domain and provide evidence of dimerization using diffusion ordered spectroscopy. The predicted secondary structure of the Cx45CT domain based on the chemical shifts identified one region of α-helical structure, which corresponds to the residues that broadened beyond detection in the dimer confirmation. Previous biophysical studies from our laboratory characterizing the CT domain from the other major cardiac connexins, Cx40 and Cx43, suggest that the amount of α-helical content may translate into the ability of a protein to dimerize. Even though the CT domain is thought to be the main regulatory domain of most connexins, the physiological role of CT dimerization is currently unknown. Therefore, these assignments will be useful for determining the intermolecular interactions that mediate Cx45CT dimerization, information that will be used to characterize dimerization in functional channels, as well as characterizing the binding sites for molecular partners involved in Cx45 regulation.

Original languageEnglish (US)
Pages (from-to)293-297
Number of pages5
JournalBiomolecular NMR Assignments
Volume7
Issue number2
DOIs
StatePublished - Oct 1 2013

Fingerprint

Dimerization
Chemical shift
Dimers
Conformations
Monomers
Connexins
Connexin 43
Cell Communication
Spectrum Analysis
Binding Sites
Spectroscopy
Tissue
Communication
Proteins

Keywords

  • Carboxyl terminus
  • Cx45
  • Dimerization
  • Gap junction
  • Intrinsically disordered protein

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

Cite this

Chemical shift assignments of the connexin45 carboxyl terminal domain : Monomer and dimer conformations. / Kopanic, Jennifer L.; Sorgen, Paul L.

In: Biomolecular NMR Assignments, Vol. 7, No. 2, 01.10.2013, p. 293-297.

Research output: Contribution to journalArticle

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