Chemical shift assignments of the connexin37 carboxyl terminal domain

Hanjun Li, Gaelle Spagnol, Tasha K. Pontifex, Janis M. Burt, Paul L Sorgen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Connexin37 (Cx37) is a gap junction protein involved in cell-to-cell communication in the vasculature and other tissues. Cx37 suppresses proliferation of vascular cells involved in tissue development and repair in vivo, as well as tumor cells. Global deletion of Cx37 in mice leads to enhanced vasculogenesis in development, as well as collateralgenesis and angiogenesis in response to injury, which together support improved tissue remodeling and recovery following ischemic injury. Here we report the 1H, 15N, and 13C resonance assignments for an important regulatory domain of Cx37, the carboxyl terminus (CT; C233-V333). The predicted secondary structure of the Cx37CT domain based on the chemical shifts is that of an intrinsically disordered protein. In the 1H–15N HSQC, N-terminal residues S254-Y259 displayed a second weaker peak and residues E261-Y266 had significant line broadening. These residues are flanked by prolines (P250, P258, P260, and P268), suggesting proline cis–trans isomerization. Overall, these assignments will be useful for identifying the binding sites for intra- and inter-molecular interactions that affect Cx37 channel activity.

Original languageEnglish (US)
Pages (from-to)137-141
Number of pages5
JournalBiomolecular NMR Assignments
Volume11
Issue number2
DOIs
StatePublished - Oct 1 2017

Fingerprint

Chemical shift
Tissue
Proline
Intrinsically Disordered Proteins
Connexins
Molecular interactions
Wounds and Injuries
Isomerization
Cell Communication
Blood Vessels
Tumors
Repair
Binding Sites
Cells
Cell Proliferation
Recovery
Communication
Neoplasms

Keywords

  • Carboxyl terminus
  • Cx37
  • Gap junction
  • Intrinsically disordered protein

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

Cite this

Chemical shift assignments of the connexin37 carboxyl terminal domain. / Li, Hanjun; Spagnol, Gaelle; Pontifex, Tasha K.; Burt, Janis M.; Sorgen, Paul L.

In: Biomolecular NMR Assignments, Vol. 11, No. 2, 01.10.2017, p. 137-141.

Research output: Contribution to journalArticle

Li, Hanjun ; Spagnol, Gaelle ; Pontifex, Tasha K. ; Burt, Janis M. ; Sorgen, Paul L. / Chemical shift assignments of the connexin37 carboxyl terminal domain. In: Biomolecular NMR Assignments. 2017 ; Vol. 11, No. 2. pp. 137-141.
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