Chemical modification enhances the inhibitory effect of regulatory subunit antisense oligodeoxynucleotide of cAMP-dependent protein kinase type I on cell proliferation

Y. S. Cho-Chung, M. V. Nesterova, E. S. Severin, S. V. Vinogradov

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The analysis of the action of antisense oligodeoxynucleotide complementary to mRNA of the type I cAMP-dependent protein kinase regulatory subunit has revealed a stable inhibitory effect of the substance on Molt-4 leukemic cell proliferation. To enhance the efficiency of the oligodeoxynucleotide action, its molecule was subjected to terminal modifications. The hydrophobic radical-effected modification at the 5'-end was carried out at the last stage of automated synthesis using undecanol. At the 3'-end, the molecule was modified with an acridine residue. The substances synthesized were significantly more potent in inhibiting thymidine incorporation in the acid-insoluble fraction, compared to the nonmodified nucleotide. Examination of the cell cycle progression of Molt-4 cells has demonstrated a considerable shift of cells from G2/M stage to G1, the process being more effective in the case of modified oligonucleotides.

Original languageEnglish (US)
Pages (from-to)767-773
Number of pages7
JournalBiochemistry international
Volume25
Issue number4
StatePublished - Dec 1 1991

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Oligodeoxyribonucleotides
Chemical modification
Cell proliferation
Cyclic AMP-Dependent Protein Kinases
Cell Proliferation
Acridines
Molecules
Oligonucleotides
Thymidine
Cell Cycle
Nucleotides
Cells
Messenger RNA
Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chemical modification enhances the inhibitory effect of regulatory subunit antisense oligodeoxynucleotide of cAMP-dependent protein kinase type I on cell proliferation. / Cho-Chung, Y. S.; Nesterova, M. V.; Severin, E. S.; Vinogradov, S. V.

In: Biochemistry international, Vol. 25, No. 4, 01.12.1991, p. 767-773.

Research output: Contribution to journalArticle

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