Characterization of viruses infecting a eukaryotic Chlorella-like green alga

Anne M. Schuster, Dwight E. Burbank, Barbara Meister, Merri P. Skrdla, Russel H. Meints, Stanley Hattman, David Swinton, James L. Van Etten

Research output: Contribution to journalArticle

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Abstract

Nineteen plaque-forming viruses of the unicellular, eukaryotic Chlorella-like green alga, strain NC64A, were isolated from various geographic regions in the United States and characterized. Like the previously described virus, PBCV-1, all of the new viruses were large polyhedrons, sensitive to chloroform, and contained large dsDNA genomes of ca. 300 kbp. All of the viral DNAs contained 5-methyldeoxycytidine which varied from 0.1 to 47% of the deoxycytidine. In addition, 10 of the viral DNAs contained N6-methyldeoxyadenosine which varied from 8.1 to 37% of the deoxyadenosine. These viruses, along with 11 previously described viruses which replicate in the same Chlorella host, were grouped into 11 classes based on at least one of the following properties: plaque size, reaction with PBCV-1 antiserum, or the nature and abundance of methylated bases in their genomic DNA.

Original languageEnglish (US)
Pages (from-to)170-177
Number of pages8
JournalVirology
Volume150
Issue number1
DOIs
StatePublished - Apr 15 1986

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Chlorella
Chlorophyta
Viruses
5-methyldeoxycytidine
Viral DNA
Deoxycytidine
Chloroform
Immune Sera
Genome
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Schuster, A. M., Burbank, D. E., Meister, B., Skrdla, M. P., Meints, R. H., Hattman, S., ... Van Etten, J. L. (1986). Characterization of viruses infecting a eukaryotic Chlorella-like green alga. Virology, 150(1), 170-177. https://doi.org/10.1016/0042-6822(86)90276-X

Characterization of viruses infecting a eukaryotic Chlorella-like green alga. / Schuster, Anne M.; Burbank, Dwight E.; Meister, Barbara; Skrdla, Merri P.; Meints, Russel H.; Hattman, Stanley; Swinton, David; Van Etten, James L.

In: Virology, Vol. 150, No. 1, 15.04.1986, p. 170-177.

Research output: Contribution to journalArticle

Schuster, AM, Burbank, DE, Meister, B, Skrdla, MP, Meints, RH, Hattman, S, Swinton, D & Van Etten, JL 1986, 'Characterization of viruses infecting a eukaryotic Chlorella-like green alga', Virology, vol. 150, no. 1, pp. 170-177. https://doi.org/10.1016/0042-6822(86)90276-X
Schuster AM, Burbank DE, Meister B, Skrdla MP, Meints RH, Hattman S et al. Characterization of viruses infecting a eukaryotic Chlorella-like green alga. Virology. 1986 Apr 15;150(1):170-177. https://doi.org/10.1016/0042-6822(86)90276-X
Schuster, Anne M. ; Burbank, Dwight E. ; Meister, Barbara ; Skrdla, Merri P. ; Meints, Russel H. ; Hattman, Stanley ; Swinton, David ; Van Etten, James L. / Characterization of viruses infecting a eukaryotic Chlorella-like green alga. In: Virology. 1986 ; Vol. 150, No. 1. pp. 170-177.
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