Characterization of viral load, viability and persistence of influenza a virus in air and on surfaces of swine production facilities

Victor Neira, Peter Rabinowitz, Aaron Rendahl, Blanca Paccha, Shawn G. Gibbs, Montserrat Torremorell

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Indirect transmission of influenza A virus (IAV) in swine is poorly understood and information is lacking on levels of environmental exposure encountered by swine and people during outbreaks of IAV in swine barns. We characterized viral load, viability and persistence of IAV in air and on surfaces during outbreaks in swine barns. IAV was detected in pigs, air and surfaces from five confirmed outbreaks with 48% (47/98) of oral fluid, 38% (32/84) of pen railing and 43% (35/82) of indoor air samples testing positive by IAV RT-PCR. IAV was isolated from air and oral fluids yielding a mixture of subtypes (H1N1, H1N2 and H3N2). Detection of IAV RNA from air was sustained during the outbreaks with maximum levels estimated between 7 and 11 days from reported onset. Our results indicate that during outbreaks of IAV in swine, aerosols and surfaces in barns contain significant levels of IAV potentially representing an exposure hazard to both swine and people.

Original languageEnglish (US)
Article numbere0146616
JournalPLoS One
Volume11
Issue number1
DOIs
StatePublished - Jan 12 2016
Externally publishedYes

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Influenza A virus
Orthomyxoviridae
viral load
Viral Load
Viruses
Swine
Air
viability
air
Disease Outbreaks
swine
barns
mouth
Railings
swine production
Fluids
Environmental Exposure
aerosols
Aerosols
Hazards

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Characterization of viral load, viability and persistence of influenza a virus in air and on surfaces of swine production facilities. / Neira, Victor; Rabinowitz, Peter; Rendahl, Aaron; Paccha, Blanca; Gibbs, Shawn G.; Torremorell, Montserrat.

In: PLoS One, Vol. 11, No. 1, e0146616, 12.01.2016.

Research output: Contribution to journalArticle

Neira, Victor ; Rabinowitz, Peter ; Rendahl, Aaron ; Paccha, Blanca ; Gibbs, Shawn G. ; Torremorell, Montserrat. / Characterization of viral load, viability and persistence of influenza a virus in air and on surfaces of swine production facilities. In: PLoS One. 2016 ; Vol. 11, No. 1.
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