Characterization of tyrosyl kinase activity in human serum

Ming-Fong Lin, P. L. Lee, G. M. Clinton

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Tyrosyl kinase activity was detected in 1.0 μl or less of human serum with the substrates angiotensin II, polyamino acid polymer Glu-Tyr (4:1), anti-pp60src IgG, and endogenous serum proteins. Most (about 84%) of the tyrosyl kinase activity was in the 100,000 x g soluble fraction from serum and a similar level of activity was found in the soluble fraction from plasma. Expression of tyrosyl kinase activity in individual serum samples differed more than 15-fold. The different levels of tyrosyl kinase activity were not likely due to phosphatases, proteases, ATPases, or kinase inhibitors and activators in serum. The normal serum and plasma tyrosyl kinase activities were not stimulated by epidermal growth factor, platelet-derived growth factor, insulin, or growth factors from fetal calf serum. Investigations of samples from patients with malignant disorders indicated that those with malignant melanoma contained the highest levels of serum tyrosyl kinase activity.

Original languageEnglish (US)
Pages (from-to)1582-1587
Number of pages6
JournalJournal of Biological Chemistry
Volume260
Issue number3
StatePublished - Jan 1 1985

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Human Activities
Phosphotransferases
Serum
Plasmas
Platelet-Derived Growth Factor
Phosphoric Monoester Hydrolases
Epidermal Growth Factor
Angiotensin II
Adenosine Triphosphatases
Blood Proteins
Melanoma
Intercellular Signaling Peptides and Proteins
Polymers
Peptide Hydrolases
Immunoglobulin G
Insulin
Acids
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of tyrosyl kinase activity in human serum. / Lin, Ming-Fong; Lee, P. L.; Clinton, G. M.

In: Journal of Biological Chemistry, Vol. 260, No. 3, 01.01.1985, p. 1582-1587.

Research output: Contribution to journalArticle

Lin, M-F, Lee, PL & Clinton, GM 1985, 'Characterization of tyrosyl kinase activity in human serum', Journal of Biological Chemistry, vol. 260, no. 3, pp. 1582-1587.
Lin, Ming-Fong ; Lee, P. L. ; Clinton, G. M. / Characterization of tyrosyl kinase activity in human serum. In: Journal of Biological Chemistry. 1985 ; Vol. 260, No. 3. pp. 1582-1587.
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