Characterization of trout galanin and its distribution in trout brain and pituitary

Isabelle Anglade, Yunxia Wang, Jorgen Jensen, Gerard Tramu, Olivier Kah, J. Michael Conlon

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Galanin was purified from an extract of the stomach of the rainbow trout, Oncorhynchus mykiss, and its primary structure was established as Gly‐Trp‐Thr‐Leu‐Asn‐Ser‐Ala‐Gly‐Tyr‐Leu10‐Leu‐Gly‐Pro‐His‐Gly‐Ile‐Asp‐Gly‐His‐Arg20‐Thr‐Leu‐Ser‐Asp‐Lys‐His‐Gly‐Leu‐Ala. Trout galanin shows six amino acid substitutions compared with pig galanin, but the N‐terminal region (residues 1–14) has been fully conserved. The distribution of galanin‐immunoreactive (GAL‐IR) structures in the trout brain and pituitary was studied via immunohistochemistry. GAL‐IR cell bodies were observed only in the caudal telencephalon, the preoptic region, and the mediobasal hypothalamus. GAL‐IR fibers, however, are widely distributed throughout the brain, with a much lower density in the midbrain and posterior brain than in the tel‐ and diencephalon. Particularly dense innervation of the mediobasal hypothalamus, the ventral and supracommissuralis parts of the caudal telencephalon, and the region above and below the anterior commissure was observed. A heavy innervation of the pituitary was consistently detected. GAL‐IR fibers were present in neurohypophyseal digitations of both the anterior and intermediate lobes with highest density in the region of the proximal pars distalis, where growth hormone and gonadotropic cells are located. Fibers were also seen in digitations of the rostral pars distalis, in particular between the prolactin follicles. The distribution of GAL‐IR neurons in the central nervous system and pituitary of the trout suggests that the peptide may exercise an important role in the regulation of neuroendocrine functions, particularly those related to reproduction. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)63-74
Number of pages12
JournalJournal of Comparative Neurology
Volume350
Issue number1
DOIs
StatePublished - Dec 1 1994

Fingerprint

Galanin
Trout
Telencephalon
Oncorhynchus mykiss
Hypothalamus
Brain
Diencephalon
Amino Acid Substitution
Mesencephalon
Prolactin
Growth Hormone
Reproduction
Stomach
Swine
Central Nervous System
Immunohistochemistry
Neurons
Peptides

Keywords

  • gonadotroph
  • neuropeptide
  • purification radioimmunoassay
  • teleost

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Characterization of trout galanin and its distribution in trout brain and pituitary. / Anglade, Isabelle; Wang, Yunxia; Jensen, Jorgen; Tramu, Gerard; Kah, Olivier; Conlon, J. Michael.

In: Journal of Comparative Neurology, Vol. 350, No. 1, 01.12.1994, p. 63-74.

Research output: Contribution to journalArticle

Anglade, Isabelle ; Wang, Yunxia ; Jensen, Jorgen ; Tramu, Gerard ; Kah, Olivier ; Conlon, J. Michael. / Characterization of trout galanin and its distribution in trout brain and pituitary. In: Journal of Comparative Neurology. 1994 ; Vol. 350, No. 1. pp. 63-74.
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