Characterization of the soluble guanylyl cyclase β-subunit gene in the mosquito Anopheles gambiae

Adalgisa Caccone, B. A. Garcia, K. D. Mathiopoulos, G. S. Min, Etsuko Moriyama, J. R. Powell

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Genomic DNA corresponding to the soluble guanylyl cyclase β-subunit (GCSβ) gene was cloned and sequenced from Anopheles gambiae. The sequence was 8103 bp long and presumably included the entire coding region. The deduced amino acid sequence was 71% and 62% similar to previously known Drosophila and vertebrate GCSβ, while the C-terminus of A. gambiae GCSβ was shorter. Because of the conserved characteristics in each functional domain, the high G+C% in the third codon positions compared to the introns, the lack of internal stop codons, and the fact that we identified the gene from a cDNA, we conclude that this A. gambiae gene is functional. This is the first detailed description of a guanylyl cyclase gene structure (e.g. intron-exon boundaries). Interestingly, within the fifth intron we found high similarity to the flanking regions of the Pegasus-27 transposable element and other noncoding regions of the A. gambiae genome.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalInsect Molecular Biology
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1999

Fingerprint

Anopheles gambiae
guanylate cyclase
Guanylate Cyclase
Culicidae
Genes
Introns
introns
genes
DNA Transposable Elements
Terminator Codon
stop codon
codons
Codon
transposons
Drosophila
functional properties
exons
Vertebrates
Amino Acid Sequence
Exons

Keywords

  • Anopheles gambiae
  • DNA
  • Guanylyl cyclase β-subunit
  • Molecular evolution
  • Pegasus transposable element

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

Characterization of the soluble guanylyl cyclase β-subunit gene in the mosquito Anopheles gambiae. / Caccone, Adalgisa; Garcia, B. A.; Mathiopoulos, K. D.; Min, G. S.; Moriyama, Etsuko; Powell, J. R.

In: Insect Molecular Biology, Vol. 8, No. 1, 01.01.1999, p. 23-30.

Research output: Contribution to journalArticle

Caccone, Adalgisa ; Garcia, B. A. ; Mathiopoulos, K. D. ; Min, G. S. ; Moriyama, Etsuko ; Powell, J. R. / Characterization of the soluble guanylyl cyclase β-subunit gene in the mosquito Anopheles gambiae. In: Insect Molecular Biology. 1999 ; Vol. 8, No. 1. pp. 23-30.
@article{b9f60fb3f5dc4c08b832a3fa58a2e643,
title = "Characterization of the soluble guanylyl cyclase β-subunit gene in the mosquito Anopheles gambiae",
abstract = "Genomic DNA corresponding to the soluble guanylyl cyclase β-subunit (GCSβ) gene was cloned and sequenced from Anopheles gambiae. The sequence was 8103 bp long and presumably included the entire coding region. The deduced amino acid sequence was 71{\%} and 62{\%} similar to previously known Drosophila and vertebrate GCSβ, while the C-terminus of A. gambiae GCSβ was shorter. Because of the conserved characteristics in each functional domain, the high G+C{\%} in the third codon positions compared to the introns, the lack of internal stop codons, and the fact that we identified the gene from a cDNA, we conclude that this A. gambiae gene is functional. This is the first detailed description of a guanylyl cyclase gene structure (e.g. intron-exon boundaries). Interestingly, within the fifth intron we found high similarity to the flanking regions of the Pegasus-27 transposable element and other noncoding regions of the A. gambiae genome.",
keywords = "Anopheles gambiae, DNA, Guanylyl cyclase β-subunit, Molecular evolution, Pegasus transposable element",
author = "Adalgisa Caccone and Garcia, {B. A.} and Mathiopoulos, {K. D.} and Min, {G. S.} and Etsuko Moriyama and Powell, {J. R.}",
year = "1999",
month = "1",
day = "1",
doi = "10.1046/j.1365-2583.1999.810023.x",
language = "English (US)",
volume = "8",
pages = "23--30",
journal = "Insect Molecular Biology",
issn = "0962-1075",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Characterization of the soluble guanylyl cyclase β-subunit gene in the mosquito Anopheles gambiae

AU - Caccone, Adalgisa

AU - Garcia, B. A.

AU - Mathiopoulos, K. D.

AU - Min, G. S.

AU - Moriyama, Etsuko

AU - Powell, J. R.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Genomic DNA corresponding to the soluble guanylyl cyclase β-subunit (GCSβ) gene was cloned and sequenced from Anopheles gambiae. The sequence was 8103 bp long and presumably included the entire coding region. The deduced amino acid sequence was 71% and 62% similar to previously known Drosophila and vertebrate GCSβ, while the C-terminus of A. gambiae GCSβ was shorter. Because of the conserved characteristics in each functional domain, the high G+C% in the third codon positions compared to the introns, the lack of internal stop codons, and the fact that we identified the gene from a cDNA, we conclude that this A. gambiae gene is functional. This is the first detailed description of a guanylyl cyclase gene structure (e.g. intron-exon boundaries). Interestingly, within the fifth intron we found high similarity to the flanking regions of the Pegasus-27 transposable element and other noncoding regions of the A. gambiae genome.

AB - Genomic DNA corresponding to the soluble guanylyl cyclase β-subunit (GCSβ) gene was cloned and sequenced from Anopheles gambiae. The sequence was 8103 bp long and presumably included the entire coding region. The deduced amino acid sequence was 71% and 62% similar to previously known Drosophila and vertebrate GCSβ, while the C-terminus of A. gambiae GCSβ was shorter. Because of the conserved characteristics in each functional domain, the high G+C% in the third codon positions compared to the introns, the lack of internal stop codons, and the fact that we identified the gene from a cDNA, we conclude that this A. gambiae gene is functional. This is the first detailed description of a guanylyl cyclase gene structure (e.g. intron-exon boundaries). Interestingly, within the fifth intron we found high similarity to the flanking regions of the Pegasus-27 transposable element and other noncoding regions of the A. gambiae genome.

KW - Anopheles gambiae

KW - DNA

KW - Guanylyl cyclase β-subunit

KW - Molecular evolution

KW - Pegasus transposable element

UR - http://www.scopus.com/inward/record.url?scp=0033081285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033081285&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2583.1999.810023.x

DO - 10.1046/j.1365-2583.1999.810023.x

M3 - Article

VL - 8

SP - 23

EP - 30

JO - Insect Molecular Biology

JF - Insect Molecular Biology

SN - 0962-1075

IS - 1

ER -