Characterization of the relationship between polysaccharide intercellular adhesin and hemagglutination in Staphylococcus epidermidis

Paul D Fey, Joseph S. Ulphani, Friedrich Götz, Christine Heilmann, Dietrich Mack, Mark Edmund Rupp

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

To determine whether a relationship exists between biofilm formation and hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and hemagglutination. Strains that produced biofilm were significantly (P <. 001) more likely to mediate hemagglutination (16 biofilm-positive/hemagglutination-positive strains and 19 biofilm- negative/hemagglutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated hemagglutination (≥1/128). It was concluded that production of PIA and hemagglutination are strongly associated and that PIA, at least in part, mediates hemagglutination in S. epidermidis.

Original languageEnglish (US)
Pages (from-to)1561-1564
Number of pages4
JournalJournal of Infectious Diseases
Volume179
Issue number6
DOIs
StatePublished - Jun 7 1999

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Staphylococcus epidermidis
Hemagglutination
Biofilms
polysaccharide intercellular adhesin
Operon
Endocarditis
Staphylococcus
Glass
Plasmids
Polymerase Chain Reaction
Skin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Characterization of the relationship between polysaccharide intercellular adhesin and hemagglutination in Staphylococcus epidermidis. / Fey, Paul D; Ulphani, Joseph S.; Götz, Friedrich; Heilmann, Christine; Mack, Dietrich; Rupp, Mark Edmund.

In: Journal of Infectious Diseases, Vol. 179, No. 6, 07.06.1999, p. 1561-1564.

Research output: Contribution to journalArticle

Fey, Paul D ; Ulphani, Joseph S. ; Götz, Friedrich ; Heilmann, Christine ; Mack, Dietrich ; Rupp, Mark Edmund. / Characterization of the relationship between polysaccharide intercellular adhesin and hemagglutination in Staphylococcus epidermidis. In: Journal of Infectious Diseases. 1999 ; Vol. 179, No. 6. pp. 1561-1564.
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