Characterization of the protein binding of chiral drugs by high-performance affinity chromatography interactions of R- and S-ibuprofen with human serum albumin

David S. Hage, Terence A.G. Noctor, Irving W. Wainer

Research output: Contribution to journalArticle

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Abstract

Zonal elution and high-performance affinity chromatography were used to study the different binding characteristics of R- and S-ibuprofen with the protein human serum albumin (HSA). This was done by injecting small amounts of R- and S-ibuprofen onto an immobilized HSA column in the presence of a mobile phase that contained a known concentration of R- or S-ibuprofen as a competing agent. These studies indicated that R- and S-ibuprofen had one common binding site on the immobilized HSA column. In addition, S-ibuprofen had at least one other major binding region. The association equilibrium constant for R-ibuprofen with HSA was found to be 5.3·105 M-1 at pH 6.9 and 25°C. Under the same conditions, the association constants for S-ibuprofen at its two sites were 1.1·105 M-1 and 1.2·105 M-1. The S-ibuprofen sites were present in about a 1:1 ratio and appeared to exhibit some allosteric interactions at high S-ibuprofen concentrations. Thee chromatographic technique used in this work is a general one which can be adapted for use in studying the interactions of other chiral compounds with either HSA or additional proteins.

Original languageEnglish (US)
Pages (from-to)23-32
Number of pages10
JournalJournal of Chromatography A
Volume693
Issue number1
DOIs
Publication statusPublished - Feb 17 1995

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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