Characterization of the maize xylem sap proteome

Sophie Alvarez, Jason Q.D. Goodger, Ellen L. Marsh, Sixue Chen, Victor S. Asirvatham, Daniel P. Schachtman

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The xylem in plants has mainly been described as a conduit for water and minerals, but emerging evidence also indicates that the xylem contains protein. To study the proteins in xylem sap, we characterized the identity and composition of the maize xylem sap proteome. The composition of the xylem sap proteome in maize revealed proteins related to different phases of xylem differentiation including cell wall metabolism, secondary cell wall synthesis, and programmed cell death. Many proteins were found to be present as multiple isoforms and some of these isoforms are glycosylated. Proteins involved in defense mechanisms were also present in xylem sap and the sap proteins were shown to have antifungal activity in bioassays.

Original languageEnglish (US)
Pages (from-to)963-972
Number of pages10
JournalJournal of proteome research
Volume5
Issue number4
DOIs
StatePublished - Apr 1 2006

Fingerprint

Xylem
Proteome
Zea mays
Proteins
Protein Isoforms
Cell Wall
Cells
Mineral Waters
Bioassay
Cell death
Chemical analysis
Metabolism
Biological Assay
Cell Death

Keywords

  • Antifungal activity
  • Corn
  • Glycoprotein
  • Isoform
  • Maize
  • Plants
  • Proteome
  • Xylem sap

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Alvarez, S., Goodger, J. Q. D., Marsh, E. L., Chen, S., Asirvatham, V. S., & Schachtman, D. P. (2006). Characterization of the maize xylem sap proteome. Journal of proteome research, 5(4), 963-972. https://doi.org/10.1021/pr050471q

Characterization of the maize xylem sap proteome. / Alvarez, Sophie; Goodger, Jason Q.D.; Marsh, Ellen L.; Chen, Sixue; Asirvatham, Victor S.; Schachtman, Daniel P.

In: Journal of proteome research, Vol. 5, No. 4, 01.04.2006, p. 963-972.

Research output: Contribution to journalArticle

Alvarez, S, Goodger, JQD, Marsh, EL, Chen, S, Asirvatham, VS & Schachtman, DP 2006, 'Characterization of the maize xylem sap proteome', Journal of proteome research, vol. 5, no. 4, pp. 963-972. https://doi.org/10.1021/pr050471q
Alvarez S, Goodger JQD, Marsh EL, Chen S, Asirvatham VS, Schachtman DP. Characterization of the maize xylem sap proteome. Journal of proteome research. 2006 Apr 1;5(4):963-972. https://doi.org/10.1021/pr050471q
Alvarez, Sophie ; Goodger, Jason Q.D. ; Marsh, Ellen L. ; Chen, Sixue ; Asirvatham, Victor S. ; Schachtman, Daniel P. / Characterization of the maize xylem sap proteome. In: Journal of proteome research. 2006 ; Vol. 5, No. 4. pp. 963-972.
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