Characterization of the Jembrana disease virus tat gene and the cis- and trans-regulatory elements in its long terminal repeats

Hexin Chen, Graham Wilcox, Gde Kertayadnya, Charles Wood

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Jembrana disease virus (JDV) is a newly identified bovine lentivirus that is closely related to the bovine immunodeficiency virus (BIV). JDV contains a tat gene, encoded by two exons, which has potent transactivation activity. Cotransfection of the JDV tat expression plasmid with the JDV promoter chloramphenicol acetyltransferase (CAT) construct pJDV-U3R resulted in a substantial increase in the level of CAT mRNA transcribed from the JDV long terminal repeat (LTR) and a dramatic increase in the CAT protein level. Deletion analysis of the LTR sequences showed that sequences spanning nucleotides -68 to +53, including the TATA box and the predicted first stem- loop structure of the predicted Tat response element (TAR), were required for efficient transactivation. The results, derived from site-directed mutagenesis experiments, suggested that the base pairing in the stem of the first stem-loop structure in the TAR region was important for JDV Tat- mediated transactivation; in contrast, nucleotide substitutions in the loop region of JDV TAR had less effect. For the JDV LTR, upstream sequences, from nucleotide -196 and beyond, as well as the predicted secondary structures in the R region, may have a negative effect on basal JDV promoter activity. Deletion of these regions resulted in a four- to fivefold increase in basal expression. The JDV Tat is also a potent transactivator of other animal and primate lentivirus promoters. It transactivated BIV and human immunodeficiency virus type 1 (HIV-1) LTRs to levels similar to those with their homologous Tat proteins. In contrast, HIV-1 Tat has minimal effects on JDV LTR expression, whereas BIV Tat moderately transactivated the JDV LTR. Our study suggests that JDV may use a mechanism of transactivation similar but not identical to those of other animal and primate lentiviruses.

Original languageEnglish (US)
Pages (from-to)658-666
Number of pages9
JournalJournal of virology
Volume73
Issue number1
StatePublished - Jan 1 1999

Fingerprint

tat Genes
terminal repeat sequences
Terminal Repeat Sequences
Virus Diseases
viruses
genes
Bovine Immunodeficiency Virus
Bovine immunodeficiency virus
transcriptional activation
chloramphenicol acetyltransferase
Lentivirus
Transcriptional Activation
Chloramphenicol O-Acetyltransferase
response elements
Response Elements
Primate Lentiviruses
promoter regions
Human immunodeficiency virus 1
Bovine Lentiviruses
stems

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Characterization of the Jembrana disease virus tat gene and the cis- and trans-regulatory elements in its long terminal repeats. / Chen, Hexin; Wilcox, Graham; Kertayadnya, Gde; Wood, Charles.

In: Journal of virology, Vol. 73, No. 1, 01.01.1999, p. 658-666.

Research output: Contribution to journalArticle

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