Characterization of the interactions of α-catenin with α-actinin and β-catenin/plakoglobin

Jill E. Nieset, Ann R. Redfield, Fang Jin, Karen A. Knudsen, Keith R. Johnson, Margaret J. Wheelock

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Abstract

Cadherins are calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. To function in cell-cell adhesion, the transmembrane cadherin molecule must be associated with the cytoskeleton via cytoplasmic proteins known as catenins. Three catenins, α-catenin, β-catenin and γ-catenin (also known as plakoglobin), have been identified. β-catenin or plakoglobin is associated directly with the cadherin; α-catenin binds to β-catenin/plakoglobin and serves to link the cadherin/catenin complex to the actin cytoskeleton. The domains on the cadherin and β-catenin/plakoglobin that are responsible for protein-protein interactions have been mapped. However, little is known about the molecular interactions between α-catenin and β-catenin/plakoglobin or about the interactions between α-catenin and the cytoskeleton. In this study we have used the yeast two-hybrid system to map the domains on α-catenin that allow it to associate with β-catenin/plakoglobin and with α-actinin. We also identify a region on α-actinin that is responsible for its interaction with α-catenin. The yeast two-hybrid data were confirmed with biochemical studies.

Original languageEnglish (US)
Pages (from-to)1013-1022
Number of pages10
JournalJournal of cell science
Volume110
Issue number8
StatePublished - Jun 3 1997

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gamma Catenin
Actinin
Catenins
Cadherins
Cytoskeleton
Cell Adhesion
Two-Hybrid System Techniques
Proteins

Keywords

  • Cadherin
  • Cytoskeleton
  • Plakoglobin
  • α-actinin
  • α-catenin
  • β-catenin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nieset, J. E., Redfield, A. R., Jin, F., Knudsen, K. A., Johnson, K. R., & Wheelock, M. J. (1997). Characterization of the interactions of α-catenin with α-actinin and β-catenin/plakoglobin. Journal of cell science, 110(8), 1013-1022.

Characterization of the interactions of α-catenin with α-actinin and β-catenin/plakoglobin. / Nieset, Jill E.; Redfield, Ann R.; Jin, Fang; Knudsen, Karen A.; Johnson, Keith R.; Wheelock, Margaret J.

In: Journal of cell science, Vol. 110, No. 8, 03.06.1997, p. 1013-1022.

Research output: Contribution to journalArticle

Nieset, JE, Redfield, AR, Jin, F, Knudsen, KA, Johnson, KR & Wheelock, MJ 1997, 'Characterization of the interactions of α-catenin with α-actinin and β-catenin/plakoglobin', Journal of cell science, vol. 110, no. 8, pp. 1013-1022.
Nieset JE, Redfield AR, Jin F, Knudsen KA, Johnson KR, Wheelock MJ. Characterization of the interactions of α-catenin with α-actinin and β-catenin/plakoglobin. Journal of cell science. 1997 Jun 3;110(8):1013-1022.
Nieset, Jill E. ; Redfield, Ann R. ; Jin, Fang ; Knudsen, Karen A. ; Johnson, Keith R. ; Wheelock, Margaret J. / Characterization of the interactions of α-catenin with α-actinin and β-catenin/plakoglobin. In: Journal of cell science. 1997 ; Vol. 110, No. 8. pp. 1013-1022.
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