Characterization of the immunophenotype and the metastatic properties of a murine T-lymphoma cell line. Unexpected expression of cytoplasmatic CD4

C. Mongini, P. Ruybal, M. J. Gravisaco, M. Croci, M. Sánchez Lockhart, V. Fabris, C. Waldner

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We report the first characterization of a mouse T-lymphoma cell line that surprisingly expresses cytoplasmatic (cy) cyCD4. Phenotypically, LBC cells are CD5+, CD8+, CD16+, CD24+, CD25+, CD2-/dim, CD3-/dim, TCRβ-/dim, TCRγδ CD154-, CD40-, and CD45R-. Coexpress cyTCRβ, cyCD3, cyCD4, and yet lack surface CD4 expression. Transplantation of LBC cells into mice resulted in an aggressive T-lymphoblastic lymphoma that infiltrated lymph nodes, thymus, spleen, liver, ovary, and uterus but not peripheral blood or bone marrow. LBC cells display a modal chromosome number of 39 and a near-diploid karyotype. Based on the characterization data, we demonstrated that the LBC cell line was derived from an early T-cell lymphocyte precursor. We propose that the malignant cell transformation of LBC cells could coincide with the transition stage from late double-negative, DN3 (CD4-, CD8-CD44-/low, CD25+) or DN4 (CD4-/low, CD8-/low, CD44-, CD25-) to double-positive (DP: CD4+CD8+) stage of T-cell development. LBC cells provide a T-lymphoblastic lymphoma model derived from a malignant early T-lymphocyte that can be potentially useful as a model to study both cellular regulation and differentiation of T-cells. In addition, LBC tumor provides a short latency neoplasm to study cellular regulation and to perform preclinical trials of lymphoma-related disorders.

Original languageEnglish (US)
Article number3708499
Pages (from-to)499-504
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume37
Issue number8
DOIs
StatePublished - Jan 1 2001

Fingerprint

Lymphoma
Cell Line
T-Lymphocytes
Precursor Cell Lymphoblastic Leukemia-Lymphoma
T-Lymphoid Precursor Cells
Cell Transplantation
Diploidy
Karyotype
Thymus Gland
Uterus
Ovary
Neoplasms
Spleen
Chromosomes
Lymph Nodes
Bone Marrow
Lymphocytes
Liver

Keywords

  • Cytoplasmatic CD3
  • Cytoplasmatic CD4
  • Cytoplasmatic TCRβ
  • Murine T-cell lymphoma
  • Phenotypic characterization
  • T-cell line

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Characterization of the immunophenotype and the metastatic properties of a murine T-lymphoma cell line. Unexpected expression of cytoplasmatic CD4. / Mongini, C.; Ruybal, P.; Gravisaco, M. J.; Croci, M.; Lockhart, M. Sánchez; Fabris, V.; Waldner, C.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 37, No. 8, 3708499, 01.01.2001, p. 499-504.

Research output: Contribution to journalArticle

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AU - Mongini, C.

AU - Ruybal, P.

AU - Gravisaco, M. J.

AU - Croci, M.

AU - Lockhart, M. Sánchez

AU - Fabris, V.

AU - Waldner, C.

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