Characterization of the human platelet/endothelial cell adhesion molecule-1 promoter: Identification of a GATA-2 binding element required for optimal transcriptional activity

Richard J. Gumina, Nancy E. Kirschbaum, Kim Piotrowski, Peter J. Newman

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Abstract

Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on platelets, endothelial cells, and certain leukocyte subsets. To examine the factors controlling vascular-specific expression of PECAM-1, we cloned the 5'-flanking region of the PECAM-1 gene and analyzed its transcriptional activity. 5'-Rapid amplification of cDNA ends (5'-RACE) analysis showed that transcription initiation occurred at several closely spaced nearby sites originating approximately 204 bp upstream from the translation start site. Analysis of the sequence immediately upstream from the transcription initiation site (TIS) showed no canonical TATA or CAAT elements, however an initiator element commonly found in TATA-less promoters encompassed the TIS. 5'-serially truncated PECAM-1 promoter segments cloned in front of a luciferase reporter drove transcription in both a lineage- and orientation-specific manner. Putative cis-acting control elements present within a 300-bp core promoter included two ets sites, an Sp1 site, tandem E-box domains, two GATA- associated sites (CACCC), an AP-2 binding site, and a GATA element at -24. Mutational analysis showed that optimal transcriptional activity required the GATA sequence at position -24, and gel-shift assays further showed that the GATA-2 transcription factor, but not GATA-1, bound to this region of the PECAM-1 promoter. Understanding the cis- and transacting factors that regulate the tissue-specific expression of PECAM-1 should increase our understanding of the mechanisms by which vascular-specific gene expression is achieved.

Original languageEnglish (US)
Pages (from-to)1260-1269
Number of pages10
JournalBlood
Volume89
Issue number4
StatePublished - Feb 15 1997

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CD31 Antigens
Transcription Initiation Site
Transcription
GATA2 Transcription Factor
Genes
E-Box Elements
GATA Transcription Factors
Immunoglobulin Genes
5' Flanking Region
Endothelial cells
Thromboplastin
Platelets
Luciferases
Gene expression
Amplification
Sequence Analysis
Blood Vessels
human ESAM protein
Assays
Leukocytes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Characterization of the human platelet/endothelial cell adhesion molecule-1 promoter : Identification of a GATA-2 binding element required for optimal transcriptional activity. / Gumina, Richard J.; Kirschbaum, Nancy E.; Piotrowski, Kim; Newman, Peter J.

In: Blood, Vol. 89, No. 4, 15.02.1997, p. 1260-1269.

Research output: Contribution to journalArticle

Gumina, Richard J. ; Kirschbaum, Nancy E. ; Piotrowski, Kim ; Newman, Peter J. / Characterization of the human platelet/endothelial cell adhesion molecule-1 promoter : Identification of a GATA-2 binding element required for optimal transcriptional activity. In: Blood. 1997 ; Vol. 89, No. 4. pp. 1260-1269.
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