Characterization of the cell-free translation products of carnation mottle virus genomic and subgenomic RNAs

J. C. Carrington, Thomas J Morris

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The in vitro translation products of carnation mottle virus (CarMV) genomic and subgenomic RNAs were analysed using a rabbit reticulocyte lysate system. Viral RNAs directed synthesis of three main polypeptides, p80, p40, and p34. p40, which was the predominant product using unfractionated virion RNA as template, was identified as coat protein based on electrophoretic mobility through SDS-polyacrylamide gels and immunoprecipitation with anti-CarMV serum. Upon size-fractionation of virion RNAs by sucrose gradient centrifugation and subsequent translational analysis, p40 was found to be encoded by subgenomic RNA, whereas p80 and p34 were synthesized from templates of genome length. p80 and p34 were found to contain common or overlapping amino acid sequences by analysis of cleavage peptides formed during proteolysis with α-chymotrypsin. These data support CarMV translational mechanisms other than those proposed in a previous study (R. Saloman, M. Bar-Joseph, H. Soreq, I. Gozes, and U. Z. Littauer, 1978,Virology 90, 288-298).

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalVirology
Volume144
Issue number1
DOIs
StatePublished - Jul 15 1985

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Carmovirus
RNA
Virion
Peptides
Virology
Reticulocytes
Protein Sequence Analysis
Viral RNA
Chymotrypsin
Capsid Proteins
Centrifugation
Immunoprecipitation
Proteolysis
Sucrose
Genome
Rabbits
Serum
adjuvant P40

ASJC Scopus subject areas

  • Virology

Cite this

Characterization of the cell-free translation products of carnation mottle virus genomic and subgenomic RNAs. / Carrington, J. C.; Morris, Thomas J.

In: Virology, Vol. 144, No. 1, 15.07.1985, p. 1-10.

Research output: Contribution to journalArticle

Carrington, J. C. ; Morris, Thomas J. / Characterization of the cell-free translation products of carnation mottle virus genomic and subgenomic RNAs. In: Virology. 1985 ; Vol. 144, No. 1. pp. 1-10.
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