Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues

B. Spaulding, D. Pan, A. Ghadersohi, G. Nielsen, S. Jensen, F. Gellert, X. Ling, M. Zhang, Adrian R Black, F. Li

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Aims: To elucidate the expression and regulation of survivin in normal tissues. Methods and results: A novel monoclonal antibody (12C4) to survivin was generated. Application of this antibody to determine survivin expression in human normal adult tissues revealed that most adult tissues do not express survivin and, where it is present, survivin is largely restricted to a small subset of epithelial cells and cells with proliferative potential such as thymus. Survivin expression among positive tissues showed individual variations, ranging from zero to < 5% positive cells in epithelial cell populations. Testis is the only human adult tissue highly expressing survivin, with 60-70% positivity in the nuclei of spermatogonia. Consistent with deregulated expression of survivin associated with oncogenesis, we found that certain ligands and transcription factors differentially modulate survivin promoter activity in cancer cells versus normal/untransformed cells. Conclusion: Deregulation of survivin transcription controls in individual epithelial cells may contribute to oncogenesis in various human adult tissues.

Original languageEnglish (US)
Pages (from-to)622-633
Number of pages12
JournalHistopathology
Volume49
Issue number6
DOIs
StatePublished - Dec 1 2006

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Monoclonal Antibodies
Epithelial Cells
Carcinogenesis
Spermatogonia
Thymus Gland
Testis
Transcription Factors
Ligands
Antibodies
Population
Neoplasms

Keywords

  • Cancer tissues
  • Human adult tissues
  • Survivin expression

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. / Spaulding, B.; Pan, D.; Ghadersohi, A.; Nielsen, G.; Jensen, S.; Gellert, F.; Ling, X.; Zhang, M.; Black, Adrian R; Li, F.

In: Histopathology, Vol. 49, No. 6, 01.12.2006, p. 622-633.

Research output: Contribution to journalArticle

Spaulding, B, Pan, D, Ghadersohi, A, Nielsen, G, Jensen, S, Gellert, F, Ling, X, Zhang, M, Black, AR & Li, F 2006, 'Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues', Histopathology, vol. 49, no. 6, pp. 622-633. https://doi.org/10.1111/j.1365-2559.2006.02556.x
Spaulding, B. ; Pan, D. ; Ghadersohi, A. ; Nielsen, G. ; Jensen, S. ; Gellert, F. ; Ling, X. ; Zhang, M. ; Black, Adrian R ; Li, F. / Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. In: Histopathology. 2006 ; Vol. 49, No. 6. pp. 622-633.
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