Characterization of SSR42, a Novel virulence factor regulatory RNA that contributes to the pathogenesis of a Staphylococcus aureus USA300 representative

John M. Morrison, Eric W. Miller, Meredith A. Benson, Francis Alonzo, Pauline Yoong, Victor J. Torres, Steven Heye Hinrichs, Paul M. Dunmana

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Staphylococcus aureus is a major human pathogen that is capable of producing an expansive repertoire of cell surface-associated and extracellular virulence factors. Herein we describe an S. aureus regulatory RNA, SSR42, which modulates the expression of approximately 80 mRNA species, including several virulence factors, in S. aureus strains UAMS-1 and USA300 (LAC) during stationary-phase growth. Mutagenesis studies revealed that SSR42 codes for an 891-nucleotide RNA molecule and that the molecule's regulatory effects are mediated by the full-length transcript. Western blotting and functional assays indicated that the regulatory effects of SSR42 correlate with biologically significant changes in corresponding protein abundances. Further, in S. aureus strain LAC, SSR42 is required for wild-type levels of erythrocyte lysis, resistance to human polymorphonuclear leukocyte killing, and pathogenesis in a murine model of skin and soft tissue infection. Taken together, our results indicate that SSR42 is a novel S. aureus regulatory RNA molecule that contributes to the organism's ability to cause disease.

Original languageEnglish (US)
Pages (from-to)2924-2938
Number of pages15
JournalJournal of bacteriology
Volume194
Issue number11
DOIs
StatePublished - Jun 1 2012

Fingerprint

Virulence Factors
Staphylococcus aureus
RNA
Soft Tissue Infections
Mutagenesis
Neutrophils
Nucleotides
Erythrocytes
Western Blotting
Messenger RNA
Skin
Growth
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Characterization of SSR42, a Novel virulence factor regulatory RNA that contributes to the pathogenesis of a Staphylococcus aureus USA300 representative. / Morrison, John M.; Miller, Eric W.; Benson, Meredith A.; Alonzo, Francis; Yoong, Pauline; Torres, Victor J.; Hinrichs, Steven Heye; Dunmana, Paul M.

In: Journal of bacteriology, Vol. 194, No. 11, 01.06.2012, p. 2924-2938.

Research output: Contribution to journalArticle

Morrison, John M. ; Miller, Eric W. ; Benson, Meredith A. ; Alonzo, Francis ; Yoong, Pauline ; Torres, Victor J. ; Hinrichs, Steven Heye ; Dunmana, Paul M. / Characterization of SSR42, a Novel virulence factor regulatory RNA that contributes to the pathogenesis of a Staphylococcus aureus USA300 representative. In: Journal of bacteriology. 2012 ; Vol. 194, No. 11. pp. 2924-2938.
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