Characterization of sphingolipids from sunflower seeds with altered fatty acid composition

Joaquín J. Salas, Jonathan E. Markham, Enrique Martínez-Force, Rafael Garcés

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10 Scopus citations

Abstract

Sphingolipids are a group of lipids that are derived from long-chain 1,3-dihydroxy-2-amino bases and that are involved in important processes in plants. Long-chain bases are usually found bound to long-chain fatty acids forming ceramides, the lipophilic moiety of the most common sphingolipid classes found in plant tissues: glucosyl-ceramides and glucosyl inositol phosphoryl-ceramides (GIPCs). The developing sunflower seed kernel is a tissue rich in sphingolipids, although, importantly, its glycerolipid composition can vary if some steps of the fatty acid synthesis are altered. Here, the sphingolipid composition of the seed from different sunflower mutants with altered fatty acid compositions was studied. The long-chain base composition and content were analyzed, and it was found to be similar in all of the mutants studied. The sphingolipid species were also determined by mass spectrometry, and some differences were found in highly saturated sunflower mutants, which contained higher levels of GIPC, ceramides, and hydroxyl-ceramides.

Original languageEnglish (US)
Pages (from-to)12486-12492
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number23
DOIs
StatePublished - Dec 14 2011

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Keywords

  • Helianthus annuus
  • Sphingolipids
  • seed kernel
  • sphingoid bases
  • sunflower mutant

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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