Characterization of sodium-calcium exchange in rabbit renal arterioles

Beth C. Fowler, Pamela K. Carmines, Lawrence D. Nelson, P. Darwin Bell

Research output: Contribution to journalArticle

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Abstract

Experiments were performed to test the hypothesis that renal arterioles exhibit Na-Ca exchange capability and that this process is regulated by protein kinase C (PKC). Glomeruli with attached arterioles were dissected from rabbit kidney and loaded with fura-2 for measurement of intracellular calcium concentration ([Ca2+](i)) using microscope-based photometry. In tissue bathed in Ringer's solution containing 150 mM Na+ and 1.5 mM Ca2+, afferent and efferent arteriolar [Ca2+](i) averaged 136 ± 6 and 154 ± 7 nM, respectively. Removal of extracellular Na+ increased afferent arteriolar [Ca2+](i) by 70 ± 7 nM, while efferent arteriolar [Ca2+](i) only increased by 39 ± 5 nM (P < 0.01 vs. afferent arteriole). These responses were inhibited by 6 mM Ni2+ and required extracellular Ca2+, but were unaffected by 10 μM diltiazem. After incubation in 500 μM ouabain, 5 μM monensin, and 5 μM nigericin, [Ca2+](i) responses to removal of extracellular Na+ were exaggerated significantly, averaging 174 ± 50 nM in afferent arterioles and 222 ± 82 nM in efferent arterioles (NS vs. afferent arterioles). Moreover, responses to removal of extracellular Na+ were enhanced by 100 nM phorbol 12-myristate 13-acetate, an effect which was blocked by PKC inhibition (25 nM K252b). These data indicate that both afferent and efferent arterioles express the Na-Ca exchanger, and that PKC activity impacts on exchange capacity in these vessels.

Original languageEnglish (US)
Pages (from-to)1856-1862
Number of pages7
JournalKidney International
Volume50
Issue number6
DOIs
StatePublished - Jan 1 1996

Fingerprint

Arterioles
Sodium
Rabbits
Calcium
Kidney
Protein Kinase C
Nigericin
Photometry
Monensin
Diltiazem
Fura-2
Ouabain
Acetates

ASJC Scopus subject areas

  • Nephrology

Cite this

Characterization of sodium-calcium exchange in rabbit renal arterioles. / Fowler, Beth C.; Carmines, Pamela K.; Nelson, Lawrence D.; Bell, P. Darwin.

In: Kidney International, Vol. 50, No. 6, 01.01.1996, p. 1856-1862.

Research output: Contribution to journalArticle

Fowler, Beth C. ; Carmines, Pamela K. ; Nelson, Lawrence D. ; Bell, P. Darwin. / Characterization of sodium-calcium exchange in rabbit renal arterioles. In: Kidney International. 1996 ; Vol. 50, No. 6. pp. 1856-1862.
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