Characterization of Pseudomonas aeruginosa bacteriophage UNL-1, a bacterial virus with a novel UV-A-inducible DNA damage reactivation phenotype

Julie J Shaffer, Lisa M. Jacobsen, John O. Schrader, Kit W. Lee, Eugene L. Martin, Tyler A. Kokjohn

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

UNL-1, a lytic virus of Pseudomonas aeruginosa, was observed to express a novel inducible DNA damage reactivation activity in UV-A-irradiated P. aeruginosa host cells. The expression of bacteriophage reactivation was quantified in hosts exposed to either UV-C or UV-A radiation. While reactivation of UV-C-damaged UNL-1 was not inducible in UV-C-irradiated host cells, an approximately 13-fold induction was observed in UV-A-irradiated host cells. When host cells were exposed to sunlight, reactivation of damaged UNL-1 virus increased eightfold. The UV-A induction of UNL-1 DNA damage reactivation was supported in hosts lacking recA gene function. This report is the first description of a recA-independent, UV-inducible virus DNA damage repair system. Our findings suggest that a combination of both host and virus DNA repair processes contribute to the persistence and sustained replication of some bacterial viruses in aquatic environments.

Original languageEnglish (US)
Pages (from-to)2606-2613
Number of pages8
JournalApplied and environmental microbiology
Volume65
Issue number6
StatePublished - Jan 1 1999

Fingerprint

Pseudomonas Phages
bacteriophage
Pseudomonas aeruginosa
reactivation
DNA damage
bacteriophages
DNA Damage
phenotype
virus
Viruses
Phenotype
DNA
viruses
damage
DNA Repair
repair
cells
aquatic environment
DNA repair
Sunlight

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Characterization of Pseudomonas aeruginosa bacteriophage UNL-1, a bacterial virus with a novel UV-A-inducible DNA damage reactivation phenotype. / Shaffer, Julie J; Jacobsen, Lisa M.; Schrader, John O.; Lee, Kit W.; Martin, Eugene L.; Kokjohn, Tyler A.

In: Applied and environmental microbiology, Vol. 65, No. 6, 01.01.1999, p. 2606-2613.

Research output: Contribution to journalArticle

Shaffer, Julie J ; Jacobsen, Lisa M. ; Schrader, John O. ; Lee, Kit W. ; Martin, Eugene L. ; Kokjohn, Tyler A. / Characterization of Pseudomonas aeruginosa bacteriophage UNL-1, a bacterial virus with a novel UV-A-inducible DNA damage reactivation phenotype. In: Applied and environmental microbiology. 1999 ; Vol. 65, No. 6. pp. 2606-2613.
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