Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1

Donald E. Ward, Ingrid M. Revet, Renu Nandakumar, Jon H. Tuttle, Willem M. De Vos, John Van der Oost, Jocelyne DiRuggiero

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication.

Original languageEnglish (US)
Pages (from-to)2561-2566
Number of pages6
JournalJournal of bacteriology
Volume184
Issue number9
DOIs
StatePublished - Apr 25 2002

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Pyrococcus
Plasmids
Single-Stranded DNA
Archaea
DNA Replication
Open Reading Frames
Sequence Analysis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Ward, D. E., Revet, I. M., Nandakumar, R., Tuttle, J. H., De Vos, W. M., Van der Oost, J., & DiRuggiero, J. (2002). Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1. Journal of bacteriology, 184(9), 2561-2566. https://doi.org/10.1128/JB.184.9.2561-2566.2002

Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1. / Ward, Donald E.; Revet, Ingrid M.; Nandakumar, Renu; Tuttle, Jon H.; De Vos, Willem M.; Van der Oost, John; DiRuggiero, Jocelyne.

In: Journal of bacteriology, Vol. 184, No. 9, 25.04.2002, p. 2561-2566.

Research output: Contribution to journalArticle

Ward, DE, Revet, IM, Nandakumar, R, Tuttle, JH, De Vos, WM, Van der Oost, J & DiRuggiero, J 2002, 'Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1', Journal of bacteriology, vol. 184, no. 9, pp. 2561-2566. https://doi.org/10.1128/JB.184.9.2561-2566.2002
Ward DE, Revet IM, Nandakumar R, Tuttle JH, De Vos WM, Van der Oost J et al. Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1. Journal of bacteriology. 2002 Apr 25;184(9):2561-2566. https://doi.org/10.1128/JB.184.9.2561-2566.2002
Ward, Donald E. ; Revet, Ingrid M. ; Nandakumar, Renu ; Tuttle, Jon H. ; De Vos, Willem M. ; Van der Oost, John ; DiRuggiero, Jocelyne. / Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1. In: Journal of bacteriology. 2002 ; Vol. 184, No. 9. pp. 2561-2566.
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