Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1

Donald E. Ward, Ingrid M. Revet, Renu Nandakumar, Jon H. Tuttle, Willem M. De Vos, John Van der Oost, Jocelyne DiRuggiero

Research output: Contribution to journalArticle

30 Scopus citations


We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication.

Original languageEnglish (US)
Pages (from-to)2561-2566
Number of pages6
JournalJournal of bacteriology
Issue number9
StatePublished - Apr 25 2002


ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Ward, D. E., Revet, I. M., Nandakumar, R., Tuttle, J. H., De Vos, W. M., Van der Oost, J., & DiRuggiero, J. (2002). Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1. Journal of bacteriology, 184(9), 2561-2566.