Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum

Erin D. Scully, Tammy Gries, Deanna L. Funnell-Harris, Zhanguo Xin, Frank A. Kovacs, Wilfred Vermerris, Scott E. Sattler

Research output: Contribution to journalArticle

Abstract

The presence of lignin reduces the quality of lignocellulosic biomass for forage materials and feedstock for biofuels. In C4 grasses, the brown midrib phenotype has been linked to mutations to genes in the monolignol biosynthesis pathway. For example, the Bmr6 gene in sorghum (Sorghum bicolor) has been previously shown to encode cinnamyl alcohol dehydrogenase (CAD), which catalyzes the final step of the monolignol biosynthesis pathway. Mutations in this gene have been shown to reduce the abundance of lignin, enhance digestibility, and improve saccharification efficiencies and ethanol yields. Nine sorghum lines harboring five different bmr6 alleles were identified in an EMS-mutagenized TILLING population. DNA sequencing of Bmr6 revealed that the majority of the mutations impacted evolutionarily conserved amino acids while three-dimensional structural modeling predicted that all of these alleles interfered with the enzyme's ability to bind with its NADPH cofactor. All of the new alleles reduced in vitro CAD activity levels and enhanced glucose yields following saccharification. Further, many of these lines were associated with higher reductions in acid detergent lignin compared to lines harboring the previously characterized bmr6-ref allele. These bmr6 lines represent new breeding tools for manipulating biomass composition to enhance forage and feedstock quality.

Original languageEnglish (US)
Pages (from-to)136-149
Number of pages14
JournalJournal of Integrative Plant Biology
Volume58
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Sorghum
Lignin
Biosynthesis
Sorghum (Poaceae)
Saccharification
lignin
Genes
Alleles
biosynthesis
cinnamyl alcohol dehydrogenase
alleles
mutation
Feedstocks
Mutation
saccharification
Biomass
feedstocks
Biofuels
forage
NADP

Keywords

  • Biofuels
  • C4 grass
  • Cinnamyl alcohol dehydrogenase (CAD)
  • EMS mutagenesis
  • Forage

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

Cite this

Scully, E. D., Gries, T., Funnell-Harris, D. L., Xin, Z., Kovacs, F. A., Vermerris, W., & Sattler, S. E. (2016). Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum. Journal of Integrative Plant Biology, 58(2), 136-149. https://doi.org/10.1111/jipb.12375

Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum. / Scully, Erin D.; Gries, Tammy; Funnell-Harris, Deanna L.; Xin, Zhanguo; Kovacs, Frank A.; Vermerris, Wilfred; Sattler, Scott E.

In: Journal of Integrative Plant Biology, Vol. 58, No. 2, 01.02.2016, p. 136-149.

Research output: Contribution to journalArticle

Scully, ED, Gries, T, Funnell-Harris, DL, Xin, Z, Kovacs, FA, Vermerris, W & Sattler, SE 2016, 'Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum', Journal of Integrative Plant Biology, vol. 58, no. 2, pp. 136-149. https://doi.org/10.1111/jipb.12375
Scully, Erin D. ; Gries, Tammy ; Funnell-Harris, Deanna L. ; Xin, Zhanguo ; Kovacs, Frank A. ; Vermerris, Wilfred ; Sattler, Scott E. / Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum. In: Journal of Integrative Plant Biology. 2016 ; Vol. 58, No. 2. pp. 136-149.
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