Characterization of metastasis-associated antigens on RAW117 lymphosarcoma cell lines

Shantaram S Joshi, John G Sharp, Hemant M. Gharpure, Kenneth W. Brunson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A syngeneic murine model system was used to study the immunobiology of metastasis. The highly malignant RAW117-H10 cell line was compared to the less malignant parental RAW117-P cell line from which it was derived, for expression of cell surface antigens. Using rabbit antisera, two major glycoprotein antigens were detected on the tumor cell surfaces. Antigen-I was uniformly dsitributed ov er the surface of these cells wheras antigen-II had a patehy, punctate distribution, Antigen-I was displayed less on RAW1 17-1110 cells than on RAW117-P cells, while the expression of the other serologically distinct antigen (antigen-II) was increased on RAW117-H10 cells compared to the less malignant parental (RAW117-P) cells. This differential antigen expression was assessed by immunodiffusion, a 125I-labeled protein-A binding assay, flow cytometry and rocket immunoelectrophoresis. Both these antigens had a molecular weight of 70000 daltons. Antigen-I bound the lectin concanavalin-A whereas antigen- II did not, suggesting that antigen-I might be the viral envelope glycoprotein gp70. The identity of antigen-II is presently unknown. Syngeneic Balb/c mice injected with highly malignant and metastatic RAW117-H10 cells coated with antiserum to antigen-I were protected from early death; this effect was mot seem with RAW1 17-1110 cells coated with amtoseri, to antigen-II. The opsonizing qualities of thes antisera may be different due to antibody to antigen-II being shed more rapidly than antibody to antigen-I.

Original languageEnglish (US)
Pages (from-to)89-104
Number of pages16
JournalClinical & Experimental Metastasis
Volume5
Issue number1
DOIs
StatePublished - Jan 1 1987

Fingerprint

Non-Hodgkin's Lymphoma
Neoplasm Metastasis
Antigens
Cell Line
Immune Sera
Surface Antigens
Glycoproteins
Immunoelectrophoresis
Antibodies
Immunodiffusion
Staphylococcal Protein A
Concanavalin A
Lectins
Protein Binding
I-antigen
Flow Cytometry
Molecular Weight
Rabbits

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Characterization of metastasis-associated antigens on RAW117 lymphosarcoma cell lines. / Joshi, Shantaram S; Sharp, John G; Gharpure, Hemant M.; Brunson, Kenneth W.

In: Clinical & Experimental Metastasis, Vol. 5, No. 1, 01.01.1987, p. 89-104.

Research output: Contribution to journalArticle

@article{95dd46f3f319497984f83a85b973b62a,
title = "Characterization of metastasis-associated antigens on RAW117 lymphosarcoma cell lines",
abstract = "A syngeneic murine model system was used to study the immunobiology of metastasis. The highly malignant RAW117-H10 cell line was compared to the less malignant parental RAW117-P cell line from which it was derived, for expression of cell surface antigens. Using rabbit antisera, two major glycoprotein antigens were detected on the tumor cell surfaces. Antigen-I was uniformly dsitributed ov er the surface of these cells wheras antigen-II had a patehy, punctate distribution, Antigen-I was displayed less on RAW1 17-1110 cells than on RAW117-P cells, while the expression of the other serologically distinct antigen (antigen-II) was increased on RAW117-H10 cells compared to the less malignant parental (RAW117-P) cells. This differential antigen expression was assessed by immunodiffusion, a 125I-labeled protein-A binding assay, flow cytometry and rocket immunoelectrophoresis. Both these antigens had a molecular weight of 70000 daltons. Antigen-I bound the lectin concanavalin-A whereas antigen- II did not, suggesting that antigen-I might be the viral envelope glycoprotein gp70. The identity of antigen-II is presently unknown. Syngeneic Balb/c mice injected with highly malignant and metastatic RAW117-H10 cells coated with antiserum to antigen-I were protected from early death; this effect was mot seem with RAW1 17-1110 cells coated with amtoseri, to antigen-II. The opsonizing qualities of thes antisera may be different due to antibody to antigen-II being shed more rapidly than antibody to antigen-I.",
author = "Joshi, {Shantaram S} and Sharp, {John G} and Gharpure, {Hemant M.} and Brunson, {Kenneth W.}",
year = "1987",
month = "1",
day = "1",
doi = "10.1007/BF00116629",
language = "English (US)",
volume = "5",
pages = "89--104",
journal = "Clinical and Experimental Metastasis",
issn = "0262-0898",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Characterization of metastasis-associated antigens on RAW117 lymphosarcoma cell lines

AU - Joshi, Shantaram S

AU - Sharp, John G

AU - Gharpure, Hemant M.

AU - Brunson, Kenneth W.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - A syngeneic murine model system was used to study the immunobiology of metastasis. The highly malignant RAW117-H10 cell line was compared to the less malignant parental RAW117-P cell line from which it was derived, for expression of cell surface antigens. Using rabbit antisera, two major glycoprotein antigens were detected on the tumor cell surfaces. Antigen-I was uniformly dsitributed ov er the surface of these cells wheras antigen-II had a patehy, punctate distribution, Antigen-I was displayed less on RAW1 17-1110 cells than on RAW117-P cells, while the expression of the other serologically distinct antigen (antigen-II) was increased on RAW117-H10 cells compared to the less malignant parental (RAW117-P) cells. This differential antigen expression was assessed by immunodiffusion, a 125I-labeled protein-A binding assay, flow cytometry and rocket immunoelectrophoresis. Both these antigens had a molecular weight of 70000 daltons. Antigen-I bound the lectin concanavalin-A whereas antigen- II did not, suggesting that antigen-I might be the viral envelope glycoprotein gp70. The identity of antigen-II is presently unknown. Syngeneic Balb/c mice injected with highly malignant and metastatic RAW117-H10 cells coated with antiserum to antigen-I were protected from early death; this effect was mot seem with RAW1 17-1110 cells coated with amtoseri, to antigen-II. The opsonizing qualities of thes antisera may be different due to antibody to antigen-II being shed more rapidly than antibody to antigen-I.

AB - A syngeneic murine model system was used to study the immunobiology of metastasis. The highly malignant RAW117-H10 cell line was compared to the less malignant parental RAW117-P cell line from which it was derived, for expression of cell surface antigens. Using rabbit antisera, two major glycoprotein antigens were detected on the tumor cell surfaces. Antigen-I was uniformly dsitributed ov er the surface of these cells wheras antigen-II had a patehy, punctate distribution, Antigen-I was displayed less on RAW1 17-1110 cells than on RAW117-P cells, while the expression of the other serologically distinct antigen (antigen-II) was increased on RAW117-H10 cells compared to the less malignant parental (RAW117-P) cells. This differential antigen expression was assessed by immunodiffusion, a 125I-labeled protein-A binding assay, flow cytometry and rocket immunoelectrophoresis. Both these antigens had a molecular weight of 70000 daltons. Antigen-I bound the lectin concanavalin-A whereas antigen- II did not, suggesting that antigen-I might be the viral envelope glycoprotein gp70. The identity of antigen-II is presently unknown. Syngeneic Balb/c mice injected with highly malignant and metastatic RAW117-H10 cells coated with antiserum to antigen-I were protected from early death; this effect was mot seem with RAW1 17-1110 cells coated with amtoseri, to antigen-II. The opsonizing qualities of thes antisera may be different due to antibody to antigen-II being shed more rapidly than antibody to antigen-I.

UR - http://www.scopus.com/inward/record.url?scp=0023126751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023126751&partnerID=8YFLogxK

U2 - 10.1007/BF00116629

DO - 10.1007/BF00116629

M3 - Article

C2 - 3103962

AN - SCOPUS:0023126751

VL - 5

SP - 89

EP - 104

JO - Clinical and Experimental Metastasis

JF - Clinical and Experimental Metastasis

SN - 0262-0898

IS - 1

ER -