Characterization of mechanical behavior of an epithelial monolayer in response to epidermal growth factor stimulation

Ruiguo Yang, Jennifer Y. Chen, Ning Xi, King Wai Chiu Lai, Chengeng Qu, Carmen Kar Man Fung, Lynn S. Penn, Jun Xi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cell signaling often causes changes in cellular mechanical properties. Knowledge of such changes can ultimately lead to insight into the complex network of cell signaling. In the current study, we employed a combination of atomic force microscopy (AFM) and quartz crystal microbalance with dissipation monitoring (QCM-D) to characterize the mechanical behavior of A431 cells in response to epidermal growth factor receptor (EGFR) signaling. From AFM, which probes the upper portion of an individual cell in a monolayer of cells, we observed increases in energy dissipation, Young's modulus, and hysteresivity. Increases in hysteresivity imply a shift toward a more fluid-like mechanical ordering state in the bodies of the cells. From QCM-D, which probes the basal area of the monolayer of cells collectively, we observed decreases in energy dissipation factor. This result suggests a shift toward a more solid-like state in the basal areas of the cells. The comparative analysis of these results indicates a regionally specific mechanical behavior of the cell in response to EGFR signaling and suggests a correlation between the time-dependent mechanical responses and the dynamic process of EGFR signaling. This study also demonstrates that a combination of AFM and QCM-D is able to provide a more complete and refined mechanical profile of the cells during cell signaling.

Original languageEnglish (US)
Pages (from-to)521-526
Number of pages6
JournalExperimental Cell Research
Volume318
Issue number5
DOIs
StatePublished - Mar 10 2012

Fingerprint

Epidermal Growth Factor
Quartz Crystal Microbalance Techniques
Atomic Force Microscopy
Epidermal Growth Factor Receptor
Elastic Modulus

Keywords

  • AFM
  • Cell adhesion
  • Cell mechanics
  • Cytoskeleton
  • EGFR signaling
  • QCM-D

ASJC Scopus subject areas

  • Cell Biology

Cite this

Characterization of mechanical behavior of an epithelial monolayer in response to epidermal growth factor stimulation. / Yang, Ruiguo; Chen, Jennifer Y.; Xi, Ning; Lai, King Wai Chiu; Qu, Chengeng; Fung, Carmen Kar Man; Penn, Lynn S.; Xi, Jun.

In: Experimental Cell Research, Vol. 318, No. 5, 10.03.2012, p. 521-526.

Research output: Contribution to journalArticle

Yang, Ruiguo ; Chen, Jennifer Y. ; Xi, Ning ; Lai, King Wai Chiu ; Qu, Chengeng ; Fung, Carmen Kar Man ; Penn, Lynn S. ; Xi, Jun. / Characterization of mechanical behavior of an epithelial monolayer in response to epidermal growth factor stimulation. In: Experimental Cell Research. 2012 ; Vol. 318, No. 5. pp. 521-526.
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