Characterization of Kaposi's sarcoma-associated herpesvirus/human herpesvirus-8 ORF57 promoter

W. Duan, S. Wang, S. Liu, Charles Wood

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) is a recently discovered human gamma herpesvirus (HHV-8) that plays an important role in Kaposi's sarcoma development. Here, we further characterize the regulation of the early HHV-8 gene, open reading frame 57 (ORF57). ORF57 is a spliced gene consisting of two exons with a 108-bp intron near the 5′ end. The ORF57 mRNA can potentially be initiated at two different start sites, and its expression can be significantly stimulated by ORF50, an HHV-8 immediate early gene. The target site for ORF50 transactivation was mapped to a 40-bp fragment compassing nt 81904 to 81943 in the ORF57 promoter. Our study on the regulation of ORF57 expression by ORF50 provides the basis for further studies on the regulation of HHV-8 lytic gene expression.

Original languageEnglish (US)
Pages (from-to)403-413
Number of pages11
JournalArchives of Virology
Volume146
Issue number2
DOIs
StatePublished - Apr 12 2001

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Human Herpesvirus 8
Open Reading Frames
Immediate-Early Genes
Recombinant DNA
Kaposi's Sarcoma
Herpesviridae
Introns
Transcriptional Activation
Exons
Gene Expression
Messenger RNA
Genes

ASJC Scopus subject areas

  • Virology

Cite this

Characterization of Kaposi's sarcoma-associated herpesvirus/human herpesvirus-8 ORF57 promoter. / Duan, W.; Wang, S.; Liu, S.; Wood, Charles.

In: Archives of Virology, Vol. 146, No. 2, 12.04.2001, p. 403-413.

Research output: Contribution to journalArticle

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