Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis

Emilie Gauliard, Scot P Ouellette, Kelsey J. Rueden, Daniel Ladant

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Chlamydiae are obligate intracellular pathogens of eukaryotes. The bacteria grow in an intracellular vesicle called an inclusion, the membrane of which is heavily modified by chlamydial proteins called Incs (Inclusion membrane proteins). Incs represent 7-10% of the genomes of Chlamydia and, given their localization at the interface between the host and the pathogen, likely play a key role in the development and pathogenesis of the bacterium. However, their functions remain largely unknown. Here, we characterized the interaction properties between various Inc proteins of C. trachomatis, using a bacterial two-hybrid (BACTH) method suitable for detecting interactions between integral membrane proteins. To validate this approach, we first examined the oligomerization properties of the well-characterized IncA protein and showed that both the cytoplasmic domain and the transmembrane region independently contribute to IncA oligomerization. We then analyzed a set of Inc proteins and identified novel interactions between these components. Two small Incs, IncF, and Ct222, were found here to interact with many other Inc proteins and may thus represent interaction nodes within the inclusion membrane. Our data suggest that the Inc proteins may assemble in the membrane of the inclusion to form specific multi-molecular complexes in an hierarchical and temporal manner. These studies will help to better define the putative functions of the Inc proteins in the infectious process of Chlamydia.

Original languageEnglish (US)
Article number13
JournalFrontiers in cellular and infection microbiology
Volume5
Issue numberFEB
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Chlamydia trachomatis
Membrane Proteins
Chlamydia
Proteins
Membranes
Bacteria
Two-Hybrid System Techniques
Protein C
Eukaryota
Genome

Keywords

  • Bacterial two-hybrid system
  • BACTH
  • Chlamydia
  • Inc protein
  • Inclusion membrane proteins
  • Protein-protein interactions

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Medicine(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis. / Gauliard, Emilie; Ouellette, Scot P; Rueden, Kelsey J.; Ladant, Daniel.

In: Frontiers in cellular and infection microbiology, Vol. 5, No. FEB, 13, 2015.

Research output: Contribution to journalArticle

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