Characterization of induced neural progenitors from skin fibroblasts by a novel combination of defined factors

Changhai Tian, Qiang Liu, Kangmu Ma, Yongxiang Wang, Qiang Chen, Randall Ambroz, David L Klinkebiel, Yuju Li, Yunlong Huang, Jianqing Ding, Jie Wu, Jialin C Zheng

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recent reports have demonstrated that somatic cells can be directly converted to other differentiated cell types through ectopic expression of sets of transcription factors, directly avoiding the transition through a pluripotent state. Our previous experiments generated induced neural progenitor-like cells (iNPCs) by a novel combination of five transcription factors (Sox2, Brn2, TLX, Bmi1 and c-Myc). Here we demonstrated that the iNPCs not only possess NPC-specific marker genes, but also have qualities of primary brain-derived NPCs (WT-NPCs), including tripotent differentiation potential, mature neuron differentiation capability and synapse formation. Importantly, the mature neurons derived from iNPCs exhibit significant physiological properties, such as potassium channel activity and generation of action potential-like spikes. These results suggest that directly reprogrammed iNPCs closely resemble WT-NPCs, which may suggest an alternative strategy to overcome the restricted proliferative and lineage potential of induced neurons (iNCs) and broaden applications of cell therapy in the treatment of neurodegenerative disorders.

Original languageEnglish (US)
Article number1345
JournalScientific reports
Volume3
DOIs
StatePublished - Mar 11 2013

Fingerprint

Stem Cells
Fibroblasts
Skin
Neurons
Transcription Factors
Potassium Channels
Cell- and Tissue-Based Therapy
Neurodegenerative Diseases
Synapses
Action Potentials
Brain
Genes
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Characterization of induced neural progenitors from skin fibroblasts by a novel combination of defined factors. / Tian, Changhai; Liu, Qiang; Ma, Kangmu; Wang, Yongxiang; Chen, Qiang; Ambroz, Randall; Klinkebiel, David L; Li, Yuju; Huang, Yunlong; Ding, Jianqing; Wu, Jie; Zheng, Jialin C.

In: Scientific reports, Vol. 3, 1345, 11.03.2013.

Research output: Contribution to journalArticle

Tian, Changhai ; Liu, Qiang ; Ma, Kangmu ; Wang, Yongxiang ; Chen, Qiang ; Ambroz, Randall ; Klinkebiel, David L ; Li, Yuju ; Huang, Yunlong ; Ding, Jianqing ; Wu, Jie ; Zheng, Jialin C. / Characterization of induced neural progenitors from skin fibroblasts by a novel combination of defined factors. In: Scientific reports. 2013 ; Vol. 3.
@article{446ee73da2da49c8a7553b5a6a37bba9,
title = "Characterization of induced neural progenitors from skin fibroblasts by a novel combination of defined factors",
abstract = "Recent reports have demonstrated that somatic cells can be directly converted to other differentiated cell types through ectopic expression of sets of transcription factors, directly avoiding the transition through a pluripotent state. Our previous experiments generated induced neural progenitor-like cells (iNPCs) by a novel combination of five transcription factors (Sox2, Brn2, TLX, Bmi1 and c-Myc). Here we demonstrated that the iNPCs not only possess NPC-specific marker genes, but also have qualities of primary brain-derived NPCs (WT-NPCs), including tripotent differentiation potential, mature neuron differentiation capability and synapse formation. Importantly, the mature neurons derived from iNPCs exhibit significant physiological properties, such as potassium channel activity and generation of action potential-like spikes. These results suggest that directly reprogrammed iNPCs closely resemble WT-NPCs, which may suggest an alternative strategy to overcome the restricted proliferative and lineage potential of induced neurons (iNCs) and broaden applications of cell therapy in the treatment of neurodegenerative disorders.",
author = "Changhai Tian and Qiang Liu and Kangmu Ma and Yongxiang Wang and Qiang Chen and Randall Ambroz and Klinkebiel, {David L} and Yuju Li and Yunlong Huang and Jianqing Ding and Jie Wu and Zheng, {Jialin C}",
year = "2013",
month = "3",
day = "11",
doi = "10.1038/srep01345",
language = "English (US)",
volume = "3",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Characterization of induced neural progenitors from skin fibroblasts by a novel combination of defined factors

AU - Tian, Changhai

AU - Liu, Qiang

AU - Ma, Kangmu

AU - Wang, Yongxiang

AU - Chen, Qiang

AU - Ambroz, Randall

AU - Klinkebiel, David L

AU - Li, Yuju

AU - Huang, Yunlong

AU - Ding, Jianqing

AU - Wu, Jie

AU - Zheng, Jialin C

PY - 2013/3/11

Y1 - 2013/3/11

N2 - Recent reports have demonstrated that somatic cells can be directly converted to other differentiated cell types through ectopic expression of sets of transcription factors, directly avoiding the transition through a pluripotent state. Our previous experiments generated induced neural progenitor-like cells (iNPCs) by a novel combination of five transcription factors (Sox2, Brn2, TLX, Bmi1 and c-Myc). Here we demonstrated that the iNPCs not only possess NPC-specific marker genes, but also have qualities of primary brain-derived NPCs (WT-NPCs), including tripotent differentiation potential, mature neuron differentiation capability and synapse formation. Importantly, the mature neurons derived from iNPCs exhibit significant physiological properties, such as potassium channel activity and generation of action potential-like spikes. These results suggest that directly reprogrammed iNPCs closely resemble WT-NPCs, which may suggest an alternative strategy to overcome the restricted proliferative and lineage potential of induced neurons (iNCs) and broaden applications of cell therapy in the treatment of neurodegenerative disorders.

AB - Recent reports have demonstrated that somatic cells can be directly converted to other differentiated cell types through ectopic expression of sets of transcription factors, directly avoiding the transition through a pluripotent state. Our previous experiments generated induced neural progenitor-like cells (iNPCs) by a novel combination of five transcription factors (Sox2, Brn2, TLX, Bmi1 and c-Myc). Here we demonstrated that the iNPCs not only possess NPC-specific marker genes, but also have qualities of primary brain-derived NPCs (WT-NPCs), including tripotent differentiation potential, mature neuron differentiation capability and synapse formation. Importantly, the mature neurons derived from iNPCs exhibit significant physiological properties, such as potassium channel activity and generation of action potential-like spikes. These results suggest that directly reprogrammed iNPCs closely resemble WT-NPCs, which may suggest an alternative strategy to overcome the restricted proliferative and lineage potential of induced neurons (iNCs) and broaden applications of cell therapy in the treatment of neurodegenerative disorders.

UR - http://www.scopus.com/inward/record.url?scp=84874642686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874642686&partnerID=8YFLogxK

U2 - 10.1038/srep01345

DO - 10.1038/srep01345

M3 - Article

C2 - 23439431

AN - SCOPUS:84874642686

VL - 3

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 1345

ER -