Characterization of human colon carcinoma cell lines isolated from a single primary tumour

M. G. Brattain, M. E. Marks, J. McCombs, W. Finely, D. E. Brattain

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines.

Original languageEnglish (US)
Pages (from-to)373-381
Number of pages9
JournalBritish journal of cancer
Volume47
Issue number3
DOIs
StatePublished - Mar 1983

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Feeder Cells
Colon
Carcinoma
Nude Mice
Cell Line
Fibroblasts
Neoplasms
Carcinoembryonic Antigen
Cellular Structures
Karyotype
Heterografts
Culture Media
Cell Membrane

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Brattain, M. G., Marks, M. E., McCombs, J., Finely, W., & Brattain, D. E. (1983). Characterization of human colon carcinoma cell lines isolated from a single primary tumour. British journal of cancer, 47(3), 373-381. https://doi.org/10.1038/bjc.1983.56

Characterization of human colon carcinoma cell lines isolated from a single primary tumour. / Brattain, M. G.; Marks, M. E.; McCombs, J.; Finely, W.; Brattain, D. E.

In: British journal of cancer, Vol. 47, No. 3, 03.1983, p. 373-381.

Research output: Contribution to journalArticle

Brattain, MG, Marks, ME, McCombs, J, Finely, W & Brattain, DE 1983, 'Characterization of human colon carcinoma cell lines isolated from a single primary tumour', British journal of cancer, vol. 47, no. 3, pp. 373-381. https://doi.org/10.1038/bjc.1983.56
Brattain, M. G. ; Marks, M. E. ; McCombs, J. ; Finely, W. ; Brattain, D. E. / Characterization of human colon carcinoma cell lines isolated from a single primary tumour. In: British journal of cancer. 1983 ; Vol. 47, No. 3. pp. 373-381.
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