Characterization of growth factor responsive lung antigen presenting cells

S. E. Gay, G. H. Chen, P. J. Christensen, R. E. Goodman, R. A. McDonald, G. B. Toews

Research output: Contribution to journalArticle

Abstract

Antigen presenting cell activity in the lung is largely attributable to dendritic cells. We postulated that an immature dendritic cell precursor might be present within the pulmonary parenchyma. Furthermore, we postulated that the immunostimulatory capacity of pulmonary dendritic cells is not static but changes over time in response to local interactions with cytokines; growth factors, and parenchyma! cells. To test these hypotheses, we have isolated two populations of low density, nonphagocytic lung cells from lung digests of Balb/c mice. These two populations differ in their la expression (la bright and la dim). Both populations were CD45 positive (85-94%) and had similar expression of B7.1 (41-46% positive cells). Significant differences existed in B7.2 expression; 81% of the la bright cells but only 50% of the la dim cells expressed B7.2. la dim and la bright cells had different responses to growth factors in long term culture (18 days). Freshly isolated la bright cells were potent immunostimulatory cells in an mixed leukocyte reaction (10,500 cpm). Freshly isolated la dim cells were poor immunostimulatory cells (351±155 cpm) but their immunostimulatory capacity increased markedly in culture with M-CSF (14,151±2157 cpm). We conclude that the la dim cell population may either represent an immature dendritic cell sub-population or, alternatively, a second growth factor resoonsive Domilation of antieen oresentinE cells.

Original languageEnglish (US)
Pages (from-to)A1482
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

Fingerprint

antigen-presenting cells
Antigen-Presenting Cells
growth factors
Intercellular Signaling Peptides and Proteins
lungs
Lung
Cells
cells
dendritic cells
Dendritic Cells
Macrophage Colony-Stimulating Factor
Cytokines
Population
immatures
Mixed Lymphocyte Culture Test
Population Density
leukocytes
cytokines

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Gay, S. E., Chen, G. H., Christensen, P. J., Goodman, R. E., McDonald, R. A., & Toews, G. B. (1996). Characterization of growth factor responsive lung antigen presenting cells. FASEB Journal, 10(6), A1482.

Characterization of growth factor responsive lung antigen presenting cells. / Gay, S. E.; Chen, G. H.; Christensen, P. J.; Goodman, R. E.; McDonald, R. A.; Toews, G. B.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996, p. A1482.

Research output: Contribution to journalArticle

Gay, SE, Chen, GH, Christensen, PJ, Goodman, RE, McDonald, RA & Toews, GB 1996, 'Characterization of growth factor responsive lung antigen presenting cells', FASEB Journal, vol. 10, no. 6, pp. A1482.
Gay SE, Chen GH, Christensen PJ, Goodman RE, McDonald RA, Toews GB. Characterization of growth factor responsive lung antigen presenting cells. FASEB Journal. 1996 Dec 1;10(6):A1482.
Gay, S. E. ; Chen, G. H. ; Christensen, P. J. ; Goodman, R. E. ; McDonald, R. A. ; Toews, G. B. / Characterization of growth factor responsive lung antigen presenting cells. In: FASEB Journal. 1996 ; Vol. 10, No. 6. pp. A1482.
@article{3b1a39c650c043b4a16289cc720b3612,
title = "Characterization of growth factor responsive lung antigen presenting cells",
abstract = "Antigen presenting cell activity in the lung is largely attributable to dendritic cells. We postulated that an immature dendritic cell precursor might be present within the pulmonary parenchyma. Furthermore, we postulated that the immunostimulatory capacity of pulmonary dendritic cells is not static but changes over time in response to local interactions with cytokines; growth factors, and parenchyma! cells. To test these hypotheses, we have isolated two populations of low density, nonphagocytic lung cells from lung digests of Balb/c mice. These two populations differ in their la expression (la bright and la dim). Both populations were CD45 positive (85-94{\%}) and had similar expression of B7.1 (41-46{\%} positive cells). Significant differences existed in B7.2 expression; 81{\%} of the la bright cells but only 50{\%} of the la dim cells expressed B7.2. la dim and la bright cells had different responses to growth factors in long term culture (18 days). Freshly isolated la bright cells were potent immunostimulatory cells in an mixed leukocyte reaction (10,500 cpm). Freshly isolated la dim cells were poor immunostimulatory cells (351±155 cpm) but their immunostimulatory capacity increased markedly in culture with M-CSF (14,151±2157 cpm). We conclude that the la dim cell population may either represent an immature dendritic cell sub-population or, alternatively, a second growth factor resoonsive Domilation of antieen oresentinE cells.",
author = "Gay, {S. E.} and Chen, {G. H.} and Christensen, {P. J.} and Goodman, {R. E.} and McDonald, {R. A.} and Toews, {G. B.}",
year = "1996",
month = "12",
day = "1",
language = "English (US)",
volume = "10",
pages = "A1482",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - Characterization of growth factor responsive lung antigen presenting cells

AU - Gay, S. E.

AU - Chen, G. H.

AU - Christensen, P. J.

AU - Goodman, R. E.

AU - McDonald, R. A.

AU - Toews, G. B.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Antigen presenting cell activity in the lung is largely attributable to dendritic cells. We postulated that an immature dendritic cell precursor might be present within the pulmonary parenchyma. Furthermore, we postulated that the immunostimulatory capacity of pulmonary dendritic cells is not static but changes over time in response to local interactions with cytokines; growth factors, and parenchyma! cells. To test these hypotheses, we have isolated two populations of low density, nonphagocytic lung cells from lung digests of Balb/c mice. These two populations differ in their la expression (la bright and la dim). Both populations were CD45 positive (85-94%) and had similar expression of B7.1 (41-46% positive cells). Significant differences existed in B7.2 expression; 81% of the la bright cells but only 50% of the la dim cells expressed B7.2. la dim and la bright cells had different responses to growth factors in long term culture (18 days). Freshly isolated la bright cells were potent immunostimulatory cells in an mixed leukocyte reaction (10,500 cpm). Freshly isolated la dim cells were poor immunostimulatory cells (351±155 cpm) but their immunostimulatory capacity increased markedly in culture with M-CSF (14,151±2157 cpm). We conclude that the la dim cell population may either represent an immature dendritic cell sub-population or, alternatively, a second growth factor resoonsive Domilation of antieen oresentinE cells.

AB - Antigen presenting cell activity in the lung is largely attributable to dendritic cells. We postulated that an immature dendritic cell precursor might be present within the pulmonary parenchyma. Furthermore, we postulated that the immunostimulatory capacity of pulmonary dendritic cells is not static but changes over time in response to local interactions with cytokines; growth factors, and parenchyma! cells. To test these hypotheses, we have isolated two populations of low density, nonphagocytic lung cells from lung digests of Balb/c mice. These two populations differ in their la expression (la bright and la dim). Both populations were CD45 positive (85-94%) and had similar expression of B7.1 (41-46% positive cells). Significant differences existed in B7.2 expression; 81% of the la bright cells but only 50% of the la dim cells expressed B7.2. la dim and la bright cells had different responses to growth factors in long term culture (18 days). Freshly isolated la bright cells were potent immunostimulatory cells in an mixed leukocyte reaction (10,500 cpm). Freshly isolated la dim cells were poor immunostimulatory cells (351±155 cpm) but their immunostimulatory capacity increased markedly in culture with M-CSF (14,151±2157 cpm). We conclude that the la dim cell population may either represent an immature dendritic cell sub-population or, alternatively, a second growth factor resoonsive Domilation of antieen oresentinE cells.

UR - http://www.scopus.com/inward/record.url?scp=33749145921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749145921&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749145921

VL - 10

SP - A1482

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -