Characterization of enterohemorrhagic Escherichia coli on veal hides and carcasses

Joseph M. Bosilevac, Rong Wang, Brandon E Luedtke, Susanne Hinkley, Tommy L. Wheeler, Mohammad Koohmaraie

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Enterohemorrhagic Escherichia coli (EHEC) are Shiga toxin-producing E. coli associated with the most severe forms of foodborne illnesses. The U.S. Department of Agriculture, Food Safety and Inspection Service has identified a higher percentage of non-O157 EHEC compared with E. coli O157:H7-positive samples collected from veal trimmings than from products produced from other cattle slaughter classes. Therefore samples were collected from hides and preevisceration carcasses at five veal processors to assess E. coli O157:H7 and non-O157 EHEC contamination during bob veal and formula-fed veal dressing procedures. E. coli O157:H7 prevalence was measured by culture isolation and found to be on 20.3% of hides and 6.7% of carcasses. In contrast, a non-O157 EHEC molecular screening assay identified 90.3% of hides and 68.2% of carcasses as positive. Only carcass samples were taken forward to culture confirmation and 38.7% yielded one or more non-O157 EHEC isolates. The recovery of an EHEC varied by plant and sample collection date; values ranged from 2.1 to 87.8% among plants and from 4.2 to 64.2% within the same plant. Three plants were resampled after changes were made to sanitary dressing procedures. Between the two collection times at the three plants, hide-To-carcass transfer of E. coli O157:H7 and non-O157 EHEC was significantly reduced. All adulterant EHEC serogroups (O26, O45, O103, O111, O121, and O145) were isolated from veal carcasses as well as four other potentially pathogenic serogroups (O5, O84, O118, and O177). Bob veal was found to have a greater culture prevalence of E. coli O157:H7 and greater positive molecular screens for non-O157 EHEC than formula-fed veal (P , 0.05), but the percentage of culture-confirmed non-O157 EHEC was not different (P > 0.05) between the two types of calves. EHEC-O26,-O111, and-O121 were found more often in bob veal (P < 0.05), whereas EHEC-O103 was found more often in formula-fed veal (P < 0.05).

Original languageEnglish (US)
Pages (from-to)136-145
Number of pages10
JournalJournal of Food Protection
Volume80
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Enterohemorrhagic Escherichia coli
veal
enterohemorrhagic Escherichia coli
Escherichia coli O157
feed formulation
Bandages
serotypes
Food Inspection
Escherichia coli O26
Food Safety and Inspection Service
Shiga-Toxigenic Escherichia coli
sampling
United States Department of Agriculture
hides and skins
Shiga toxin-producing Escherichia coli
Foodborne Diseases
Food Safety
adulterated products
foodborne illness
pruning

Keywords

  • Enterohemorrhagic Escherichia coli
  • Escherichia coli O157:H7
  • Harvest
  • Non-O157 Shiga toxin-producing Escherichia coli
  • Veal

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Characterization of enterohemorrhagic Escherichia coli on veal hides and carcasses. / Bosilevac, Joseph M.; Wang, Rong; Luedtke, Brandon E; Hinkley, Susanne; Wheeler, Tommy L.; Koohmaraie, Mohammad.

In: Journal of Food Protection, Vol. 80, No. 1, 01.01.2017, p. 136-145.

Research output: Contribution to journalArticle

Bosilevac, Joseph M. ; Wang, Rong ; Luedtke, Brandon E ; Hinkley, Susanne ; Wheeler, Tommy L. ; Koohmaraie, Mohammad. / Characterization of enterohemorrhagic Escherichia coli on veal hides and carcasses. In: Journal of Food Protection. 2017 ; Vol. 80, No. 1. pp. 136-145.
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abstract = "Enterohemorrhagic Escherichia coli (EHEC) are Shiga toxin-producing E. coli associated with the most severe forms of foodborne illnesses. The U.S. Department of Agriculture, Food Safety and Inspection Service has identified a higher percentage of non-O157 EHEC compared with E. coli O157:H7-positive samples collected from veal trimmings than from products produced from other cattle slaughter classes. Therefore samples were collected from hides and preevisceration carcasses at five veal processors to assess E. coli O157:H7 and non-O157 EHEC contamination during bob veal and formula-fed veal dressing procedures. E. coli O157:H7 prevalence was measured by culture isolation and found to be on 20.3{\%} of hides and 6.7{\%} of carcasses. In contrast, a non-O157 EHEC molecular screening assay identified 90.3{\%} of hides and 68.2{\%} of carcasses as positive. Only carcass samples were taken forward to culture confirmation and 38.7{\%} yielded one or more non-O157 EHEC isolates. The recovery of an EHEC varied by plant and sample collection date; values ranged from 2.1 to 87.8{\%} among plants and from 4.2 to 64.2{\%} within the same plant. Three plants were resampled after changes were made to sanitary dressing procedures. Between the two collection times at the three plants, hide-To-carcass transfer of E. coli O157:H7 and non-O157 EHEC was significantly reduced. All adulterant EHEC serogroups (O26, O45, O103, O111, O121, and O145) were isolated from veal carcasses as well as four other potentially pathogenic serogroups (O5, O84, O118, and O177). Bob veal was found to have a greater culture prevalence of E. coli O157:H7 and greater positive molecular screens for non-O157 EHEC than formula-fed veal (P , 0.05), but the percentage of culture-confirmed non-O157 EHEC was not different (P > 0.05) between the two types of calves. EHEC-O26,-O111, and-O121 were found more often in bob veal (P < 0.05), whereas EHEC-O103 was found more often in formula-fed veal (P < 0.05).",
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