Characterization of DNA sequences that confer complement resistance in Leishmania chagasi

Rebecca R. Dahlin-Laborde, Eric J. Scolaro, Nathan M. Romine, Amanda E. Ramer-Tait, Soi Meng Lei, Jeffrey K. Beetham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Serial passage of axenically cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into high-passage (constitutively CML-sensitive) L. chagasi promastigotes; (2) transformants were screened for acquisition of CML-resistance; (3) multiple cosmid-transfectants exhibited partial CML resistance; and (4) the sequence for one of the cosmids (Cosmid 51) was determined. This report extends the analysis of Cosmid 51, and identifies by deletion analysis a subregion of the cosmid insert that is critical to the CML-resistance phenotype of Cosmid 51 transformants. We also report the sequence determination and initial CML-resistance activity of another cosmid that also confers partial resistance to CML.

Original languageEnglish (US)
Title of host publicationAnimal Biodiversity and Emerging Diseases Prediction and Prevention
PublisherBlackwell Publishing Inc.
Pages347-351
Number of pages5
ISBN (Print)9781573317146
DOIs
StatePublished - Dec 2008

Publication series

NameAnnals of the New York Academy of Sciences
Volume1149
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Leishmania infantum
Cosmids
DNA sequences
Serial Passage
Psychodidae
Genomic Library
Sequence Analysis
Genes
Phenotype
DNA

Keywords

  • Complement
  • Immunity
  • Leishmania
  • Leishmania chagasi
  • Protozoa

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Dahlin-Laborde, R. R., Scolaro, E. J., Romine, N. M., Ramer-Tait, A. E., Lei, S. M., & Beetham, J. K. (2008). Characterization of DNA sequences that confer complement resistance in Leishmania chagasi. In Animal Biodiversity and Emerging Diseases Prediction and Prevention (pp. 347-351). (Annals of the New York Academy of Sciences; Vol. 1149). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1428.099

Characterization of DNA sequences that confer complement resistance in Leishmania chagasi. / Dahlin-Laborde, Rebecca R.; Scolaro, Eric J.; Romine, Nathan M.; Ramer-Tait, Amanda E.; Lei, Soi Meng; Beetham, Jeffrey K.

Animal Biodiversity and Emerging Diseases Prediction and Prevention. Blackwell Publishing Inc., 2008. p. 347-351 (Annals of the New York Academy of Sciences; Vol. 1149).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Dahlin-Laborde, RR, Scolaro, EJ, Romine, NM, Ramer-Tait, AE, Lei, SM & Beetham, JK 2008, Characterization of DNA sequences that confer complement resistance in Leishmania chagasi. in Animal Biodiversity and Emerging Diseases Prediction and Prevention. Annals of the New York Academy of Sciences, vol. 1149, Blackwell Publishing Inc., pp. 347-351. https://doi.org/10.1196/annals.1428.099
Dahlin-Laborde RR, Scolaro EJ, Romine NM, Ramer-Tait AE, Lei SM, Beetham JK. Characterization of DNA sequences that confer complement resistance in Leishmania chagasi. In Animal Biodiversity and Emerging Diseases Prediction and Prevention. Blackwell Publishing Inc. 2008. p. 347-351. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1428.099
Dahlin-Laborde, Rebecca R. ; Scolaro, Eric J. ; Romine, Nathan M. ; Ramer-Tait, Amanda E. ; Lei, Soi Meng ; Beetham, Jeffrey K. / Characterization of DNA sequences that confer complement resistance in Leishmania chagasi. Animal Biodiversity and Emerging Diseases Prediction and Prevention. Blackwell Publishing Inc., 2008. pp. 347-351 (Annals of the New York Academy of Sciences).
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