Characterization of DNA sequences that confer complement resistance in Leishmania chagasi

Rebecca R. Dahlin-Laborde, Eric J. Scolaro, Nathan M. Romine, Amanda E. Ramer-Tait, Soi Meng Lei, Jeffrey K. Beetham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Serial passage of axenically cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into high-passage (constitutively CML-sensitive) L. chagasi promastigotes; (2) transformants were screened for acquisition of CML-resistance; (3) multiple cosmid-transfectants exhibited partial CML resistance; and (4) the sequence for one of the cosmids (Cosmid 51) was determined. This report extends the analysis of Cosmid 51, and identifies by deletion analysis a subregion of the cosmid insert that is critical to the CML-resistance phenotype of Cosmid 51 transformants. We also report the sequence determination and initial CML-resistance activity of another cosmid that also confers partial resistance to CML.

Original languageEnglish (US)
Title of host publicationAnimal Biodiversity and Emerging Diseases Prediction and Prevention
PublisherBlackwell Publishing Inc.
Pages347-351
Number of pages5
ISBN (Print)9781573317146
DOIs
Publication statusPublished - Dec 2008

Publication series

NameAnnals of the New York Academy of Sciences
Volume1149
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

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Keywords

  • Complement
  • Immunity
  • Leishmania
  • Leishmania chagasi
  • Protozoa

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Dahlin-Laborde, R. R., Scolaro, E. J., Romine, N. M., Ramer-Tait, A. E., Lei, S. M., & Beetham, J. K. (2008). Characterization of DNA sequences that confer complement resistance in Leishmania chagasi. In Animal Biodiversity and Emerging Diseases Prediction and Prevention (pp. 347-351). (Annals of the New York Academy of Sciences; Vol. 1149). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1428.099