Characterization of different crystal forms of the α-glucosidase MalA from Sulfolobus solfataricus

Heidi Asschenfeldt Ernst, Martin Willemoës, Leila Lo Leggio, Gordon Leonard, Paul Blum, Sine Larsen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

MalA is an α-glucosidase from the hyperthermophilic archaeon Sulfolobus solfataricus. It belongs to glycoside hydrolase family 31, which includes several medically interesting α-glucosidases. MalA and its selenomethionine derivative have been overproduced in Escherichia coli and crystallized in four different crystal forms. Microseeding was essential for the formation of good-quality crystals of forms 2 and 4. For three of the crystal forms (2, 3 and 4) full data sets could be collected. The most suitable crystals for structure determination are the monoclinic form 4 crystals, belonging to space group P21, from which data sets extending to 2.5 Å resolution have been collected. Self-rotation functions calculated for this form and for the orthorhombic (P212121) form 2 indicate the presence of six molecules in the asymmetric unit related by 32 symmetry.

Original languageEnglish (US)
Pages (from-to)1039-1042
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number12
DOIs
StatePublished - Dec 1 2005

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Sulfolobus solfataricus
Glucosidases
Selenomethionine
Crystals
Glycoside Hydrolases
Archaea
crystals
Escherichia coli
glucosides
Escherichia
Datasets
Derivatives
Molecules
symmetry
molecules

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Characterization of different crystal forms of the α-glucosidase MalA from Sulfolobus solfataricus. / Ernst, Heidi Asschenfeldt; Willemoës, Martin; Lo Leggio, Leila; Leonard, Gordon; Blum, Paul; Larsen, Sine.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 12, 01.12.2005, p. 1039-1042.

Research output: Contribution to journalArticle

Ernst, Heidi Asschenfeldt ; Willemoës, Martin ; Lo Leggio, Leila ; Leonard, Gordon ; Blum, Paul ; Larsen, Sine. / Characterization of different crystal forms of the α-glucosidase MalA from Sulfolobus solfataricus. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2005 ; Vol. 61, No. 12. pp. 1039-1042.
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