Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system

Yanping Zhang, Michael Nelson, Joseph W. Nietfeldt, Dwight E. Burbank, James L Van Etten

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlike NlaIII, which cleaves after the G and does not cleave either CmATG or mCATG sequences, CviAII cleaves between the C and A and is unaffected by mCATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.

Original languageEnglish (US)
Pages (from-to)5351-5356
Number of pages6
JournalNucleic acids research
Volume20
Issue number20
DOIs
StatePublished - Oct 25 1992

Fingerprint

DNA Restriction-Modification Enzymes
Chlorella
Viruses
Virus
Terminator Codon
Genes
DNA Restriction Enzymes
Methyltransferases
Restriction
Gene
vpr Genes
DNA
Endonucleases
Adenine
Methylation
DNA sequences
DNA Sequence
Termination
Overlap
Tail

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system. / Zhang, Yanping; Nelson, Michael; Nietfeldt, Joseph W.; Burbank, Dwight E.; Van Etten, James L.

In: Nucleic acids research, Vol. 20, No. 20, 25.10.1992, p. 5351-5356.

Research output: Contribution to journalArticle

Zhang, Yanping ; Nelson, Michael ; Nietfeldt, Joseph W. ; Burbank, Dwight E. ; Van Etten, James L. / Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system. In: Nucleic acids research. 1992 ; Vol. 20, No. 20. pp. 5351-5356.
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